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Methods for Transfecting Cells with Nucleic Acids of Animal Viruses: A Review: 16 (Experientia Supplementum) - Softcover
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Transfection, which is the infection of a cell with naked viral nucleic acid with the consequent production of complete virus, was first reported in 1956 for the ribonucleic acid of tobacco mosaic virus. Many reports of transfection, and several reviews of the field, have appeared since then. Crucial for the demonstration of transfection is that the viral nucleic acid is not damaged in the process of obtaining it from the virions, or from the infected tissue. To this end, procedures are designed to minimize the possibili ties of degradation of the viral nucleic acid by nucleases present in the biolo gical source. The most common method for preparing viral nucleic acid is the phenol method in which virus or infected-tissue preparations are extracted with phenol. Much of the protein goes down into the phenol phase, whereas the viral nucleic acid stays up in the aqueous phase. The transfection methods for animal virus nucleic acids are of three major kinds: (a) hypertonic methods; (b) insoluble facilitator methods; and (c) polycation methods. These methods have wide applicability to vertebrate celli animal virus NA systems, but anyone method does not seem to be highly effective for all such systems with the possible exception of the method using the polycation diethylaminoethyl-dextran. The insoluble facilitator method shows astonishing ’cell-specificity’; that is, it is a very effective method for transfecting some kinds of vertebrate cells, but nearly ineffective for some other kinds.
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Transfection, which is the infection of a cell with naked viral nucleic acid with the consequent production of complete virus, was first reported in 1956 for the ribonucleic acid of tobacco mosaic virus. Many reports of transfection, and several reviews of the field, have appeared since then. Crucial for the demonstration of transfection is that the viral nucleic acid is not damaged in the process of obtaining it from the virions, or from the infected tissue. To this end, procedures are designed to minimize the possibili ties of degradation of the viral nucleic acid by nucleases present in the biolo gical source. The most common method for preparing viral nucleic acid is the phenol method in which virus or infected-tissue preparations are extracted with phenol. Much of the protein goes down into the phenol phase, whereas the viral nucleic acid stays up in the aqueous phase. The transfection methods for animal virus nucleic acids are of three major kinds: (a) hypertonic methods; (b) insoluble facilitator methods; and (c) polycation methods. These methods have wide applicability to vertebrate celli animal virus NA systems, but anyone method does not seem to be highly effective for all such systems with the possible exception of the method using the polycation diethylaminoethyl-dextran. The insoluble facilitator method shows astonishing 'cell-specificity'; that is, it is a very effective method for transfecting some kinds of vertebrate cells, but nearly ineffective for some other kinds.
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Methods for Transfecting Cells with Nucleic Acids of Animal Viruses: a Review (Experientia Supplementum, 16) (German Edition)
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Methods for Transfecting Cells with Nucleic Acids of Animal Viruses: a Review (Experientia Supplementum, 16) (German Edition)
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Methods for Transfecting Cells with Nucleic Acids of Animal Viruses: a Review (Experientia Supplementum, 16) (German Edition)
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Methods for Transfecting Cells with Nucleic Acids of Animal Viruses
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Methods for Transfecting Cells with Nucleic Acids of Animal Viruses: a Review
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Springer, Basel, Birkhäuser Basel, Birkhäuser Aug 2014, 2014
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Taschenbuch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Transfection, which is the infection of a cell with naked viral nucleic acid with the consequent production of complete virus, was first reported in 1956 for the ribonucleic acid of tobacco mosaic virus. Many reports of transfection, and several reviews of the field, have appeared since then. Crucial for the demonstration of transfection is that the viral nucleic acid is not damaged in the process of obtaining it from the virions, or from the infected tissue. To this end, procedures are designed to minimize the possibili ties of degradation of the viral nucleic acid by nucleases present in the biolo gical source. The most common method for preparing viral nucleic acid is the phenol method in which virus or infected-tissue preparations are extracted with phenol. Much of the protein goes down into the phenol phase, whereas the viral nucleic acid stays up in the aqueous phase. The transfection methods for animal virus nucleic acids are of three major kinds: (a) hypertonic methods; (b) insoluble facilitator methods; and (c) polycation methods. These methods have wide applicability to vertebrate celli animal virus NA systems, but anyone method does not seem to be highly effective for all such systems with the possible exception of the method using the polycation diethylaminoethyl-dextran. The insoluble facilitator method shows astonishing 'cell-specificity'; that is, it is a very effective method for transfecting some kinds of vertebrate cells, but nearly ineffective for some other kinds. 82 pp. Deutsch. Bestandsnummer des Verkäufers 9783034857741
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Methods for Transfecting Cells with Nucleic Acids of Animal Viruses
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Methods for Transfecting Cells with Nucleic Acids of Animal Viruses
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Methods for Transfecting Cells With Nucleic Acids of Animal Viruses: A Review
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Methods for Transfecting Cells with Nucleic Acids of Animal Viruses: a Review
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Kartoniert / Broschiert. Zustand: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. and Review of Reviews.- Methods for Obtaining Infective Nucleic Acids.- Methods for Transfecting Cells with Nucleic Acids of Animal Viruses.- Comparisons of the Transfection Methods.- Combinations of the Transfection Methods.- Host Range.- Interference.- Ph. Bestandsnummer des Verkäufers 4318631
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Methods for Transfecting Cells with Nucleic Acids of Animal Viruses: a Review
Verlag:
Birkhäuser Basel, Springer Basel Aug 2014, 2014
ISBN 10: 3034857748
ISBN 13: 9783034857741
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Taschenbuch. Zustand: Neu. Neuware -Transfection, which is the infection of a cell with naked viral nucleic acid with the consequent production of complete virus, was first reported in 1956 for the ribonucleic acid of tobacco mosaic virus. Many reports of transfection, and several reviews of the field, have appeared since then. Crucial for the demonstration of transfection is that the viral nucleic acid is not damaged in the process of obtaining it from the virions, or from the infected tissue. To this end, procedures are designed to minimize the possibili ties of degradation of the viral nucleic acid by nucleases present in the biolo gical source. The most common method for preparing viral nucleic acid is the phenol method in which virus or infected-tissue preparations are extracted with phenol. Much of the protein goes down into the phenol phase, whereas the viral nucleic acid stays up in the aqueous phase. The transfection methods for animal virus nucleic acids are of three major kinds: (a) hypertonic methods; (b) insoluble facilitator methods; and (c) polycation methods. These methods have wide applicability to vertebrate celli animal virus NA systems, but anyone method does not seem to be highly effective for all such systems with the possible exception of the method using the polycation diethylaminoethyl-dextran. The insoluble facilitator method shows astonishing 'cell-specificity'; that is, it is a very effective method for transfecting some kinds of vertebrate cells, but nearly ineffective for some other kinds.Springer Basel AG in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 84 pp. Deutsch. Bestandsnummer des Verkäufers 9783034857741
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