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Methods of Preparation for Electron Microscopy: An Introduction for the Biomedical Sciences - Softcover
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Book by Robinson David G Ehlers Ulrich Herken Rainer Herrm
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In 1939, when the electron optics laboratory of Siemens & Halske Inc. began to manufacture the first electron microscopes, the biological and medical profes sions had an unexpected instrument at their disposal which exceeded the reso lution of the light microscope by more than a hundredfold. The immediate and broad application of this new tool was complicated by the overwhelming prob lems inherent in specimen preparation for the investigation of cellular struc tures. The microtechniques applied in light microscopy were no longer appli cable, since even the thinnest paraffin layers could not be penetrated by electrons. Many competent biological and medical research workers expressed their anxiety that objects in high vacuum would be modified due to complete dehydration and the absorbed electron energy would eventually cause degrada tion to rudimentary carbon backbones. It also seemed questionable as to whether it would be possible to prepare thin sections of approximately 0. 5 11m from heterogeneous biological specimens. Thus one was suddenly in posses sion of a completely unique instrument which, when compared with the light microscope, allowed a 10-100-fold higher resolution, yet a suitable preparation methodology was lacking. This sceptical attitude towards the application of electron microscopy in bi ology and medicine was supported simultaneously by the general opinion of colloid chemists, who postulated that in the submicroscopic region of living structures no stable building blocks existed which could be revealed with this apparatus.
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- VerlagSpringer
- Erscheinungsdatum1987
- ISBN 10 354017592X
- ISBN 13 9783540175926
- EinbandTapa blanda
- Anzahl der Seiten212
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Methods of Preparation for Electron Microscopy: An Introduction for the Biomedical Sciences by Robinson, David G., Ehlers, Ulrich, Herken, Rainer, Herrmann, Bernd, Mayer, Frank, Schürmann, Friedrich-Wilhelm [Paperback ]
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Methods of Preparation for Electron Microscopy
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Springer Berlin Heidelberg Jun 1987
(1987)
ISBN 10: 354017592X
ISBN 13: 9783540175926
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Buchbeschreibung Taschenbuch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -In 1939, when the electron optics laboratory of Siemens & Halske Inc. began to manufacture the first electron microscopes, the biological and medical profes sions had an unexpected instrument at their disposal which exceeded the reso lution of the light microscope by more than a hundredfold. The immediate and broad application of this new tool was complicated by the overwhelming prob lems inherent in specimen preparation for the investigation of cellular struc tures. The microtechniques applied in light microscopy were no longer appli cable, since even the thinnest paraffin layers could not be penetrated by electrons. Many competent biological and medical research workers expressed their anxiety that objects in high vacuum would be modified due to complete dehydration and the absorbed electron energy would eventually cause degrada tion to rudimentary carbon backbones. It also seemed questionable as to whether it would be possible to prepare thin sections of approximately 0. 5 11m from heterogeneous biological specimens. Thus one was suddenly in posses sion of a completely unique instrument which, when compared with the light microscope, allowed a 10-100-fold higher resolution, yet a suitable preparation methodology was lacking. This sceptical attitude towards the application of electron microscopy in bi ology and medicine was supported simultaneously by the general opinion of colloid chemists, who postulated that in the submicroscopic region of living structures no stable building blocks existed which could be revealed with this apparatus. 212 pp. Englisch. Bestandsnummer des Verkäufers 9783540175926
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Methods of Preparation for Electron Microscopy
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ISBN 13: 9783540175926
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Methods of Preparation for Electron Microscopy : An Introduction for the Biomedical Sciences
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Springer Berlin Heidelberg
(1987)
ISBN 10: 354017592X
ISBN 13: 9783540175926
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Buchbeschreibung Taschenbuch. Zustand: Neu. Druck auf Anfrage Neuware - Printed after ordering - In 1939, when the electron optics laboratory of Siemens & Halske Inc. began to manufacture the first electron microscopes, the biological and medical profes sions had an unexpected instrument at their disposal which exceeded the reso lution of the light microscope by more than a hundredfold. The immediate and broad application of this new tool was complicated by the overwhelming prob lems inherent in specimen preparation for the investigation of cellular struc tures. The microtechniques applied in light microscopy were no longer appli cable, since even the thinnest paraffin layers could not be penetrated by electrons. Many competent biological and medical research workers expressed their anxiety that objects in high vacuum would be modified due to complete dehydration and the absorbed electron energy would eventually cause degrada tion to rudimentary carbon backbones. It also seemed questionable as to whether it would be possible to prepare thin sections of approximately 0. 5 11m from heterogeneous biological specimens. Thus one was suddenly in posses sion of a completely unique instrument which, when compared with the light microscope, allowed a 10-100-fold higher resolution, yet a suitable preparation methodology was lacking. This sceptical attitude towards the application of electron microscopy in bi ology and medicine was supported simultaneously by the general opinion of colloid chemists, who postulated that in the submicroscopic region of living structures no stable building blocks existed which could be revealed with this apparatus. Bestandsnummer des Verkäufers 9783540175926
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Buchbeschreibung Paperback. Zustand: Brand New. 212 pages. 9.40x6.30x0.40 inches. In Stock. Bestandsnummer des Verkäufers x-354017592X
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Methods of Preparation for Electron Micros
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Buchbeschreibung Zustand: New. Translator(s): Robinson, David G. Num Pages: 190 pages, biography. BIC Classification: PDND. Category: (P) Professional & Scholarly; (U) Tertiary Education (US: College). Dimension: 244 x 170 x 11. Weight in Grams: 347. . 1987. Softcover reprint of the original 1st ed. 1987. Paperback. . . . . Bestandsnummer des Verkäufers V9783540175926
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