Rapid Cycle Real-Time PCR ― Methods and Applications: Genetics and Oncology - Hardcover

 
9783540426004: Rapid Cycle Real-Time PCR ― Methods and Applications: Genetics and Oncology
Hardcover
ISBN 10: 3540426000 ISBN 13: 9783540426004
Verlag: Springer, 2002

Inhaltsangabe

Rapid-Cycle Real-Time PCR is a powerful technique for nucleic acid amplification and analysis that often requires less than half an hour to perform. Samples are amplified by rapid-cycle PCR followed by immediate melting curve analysis in the same instrument. Melting curve analysis of PCR products with SYBR Green I often allows product identification without gel electrophoresis. Furthermore, in the presence of fluorescent hybridization probes, melting curves provide "dynamic dot blots" for fine sequence analysis, including single nucleotide polymorphisms (SNPs). The method is often cited as the most versatile, efficient method for nucleic acid analysis in research and diagnostics in the fields of genetics and oncology. Molecular diagnostics has never been easier!

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Rapid Cycle Real-Time PCR is a powerful technique for nucleic acid amplification and analysis that often requires less than half an hour to perform. Samples are amplified by rapid-cycle PCR followed by immediate melting curve analysis in the same instrument. Melting curve analysis of PCR products with SYBR Green I allow product identification without gel electrophoresis. Furthermore, in the presence of fluorescent hybridization probes, melting curves provide "dynamic dot blots" for fine sequence analysis, including single nucleotide polymorphisms. The method is often cited as the most versatile, efficient method for nucleic acid analysis in research and analysis in the fields of Genetics and Oncology. Molecular analysis has never been easier!

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Verlag
Springer
Erscheinungsdatum
2002
Sprache
Englisch
ISBN 10 
3540426000
ISBN 13 
9783540426004
Einband
Gebundene Ausgabe
Auflage
1
Anzahl der Seiten
213
Herausgeber
Dietmaier W., Wittwer C., Sivasubramanian N.
Kontakt zum Hersteller
Nicht verfügbar
Verantwortliche Person
Nicht verfügbar

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9783642639654: Rapid Cycle Real-Time PCR — Methods and Applications: Genetics and Oncology

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Softcover