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Quantitation of mRNA by Polymerase Chain Reaction: Nonradioactive PCR Methods (Springer Lab Manual) - Hardcover

Köhler, Thomas; Laßner, Dirk; Rost, Anne-Katrin; Thamm, Barbara; Pustowoit, Barbara; Remke, Harald

 
9783540591924: Quantitation of mRNA by Polymerase Chain Reaction: Nonradioactive PCR Methods (Springer Lab Manual)
Hardcover
ISBN 10: 3540591923 ISBN 13: 9783540591924
Verlag: Springer, 1995

Inhaltsangabe

In this laboratory "cook-book", the authors provide a concise guide to PCR-based techniques to quantify nucleic acids in biological and clinical samples using exclusively nonradioactive detection methods, e.g. HPLC, biotin and digoxigenin based protocols. Each method presentation also includes sections on theory, reagents, standards, applicability, limitations, and trouble shooting. In addition to the protocols, the authors also provide the necessary information on: general aspects of nucleic acid quantitation; design of PCR standards; mRNA purification; cDNA synthesis; solution hybridization; DNA sequencing. This laboratory guide enables professionals as well as beginners to adopt easily quantitative PCR protocols into their own clinical or biomedical research.

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In this laboratory "cook-book", the authors provide a concise guide to PCR-based techniques for quantifying nucleic acids in biological and clincial samples using exclusively nonradioactive detection methods, e.g. HPLC, biotin and digoxigenin based protocols. Each method presentation also includes sections on theory, reagents, standards, applicability, limitations, and trouble shooting. In addition to the protocols, the authors also provide the necessary information on: general aspects of nucleic acid quantitation; design of PCR standards; mRNA purification; cDNA synthesis; solution hybridization; DNA sequencing. This laboratory guide enables professionals as well as beginners to adopt easily quanatitative PCR protocols into their own clinical or biomedical research.

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Verlag
Springer
Erscheinungsdatum
1995
Sprache
Englisch
ISBN 10 
3540591923
ISBN 13 
9783540591924
Einband
Gebundene Ausgabe
Anzahl der Seiten
186
Kontakt zum Hersteller
Nicht verfügbar
Verantwortliche Person
CMN Printpool Inh. Mathis Neumann
https://www.also.com/ec/cms5/en_6000/6000/index.jsp

Friedrich-Karl-Str. 9
Hamburg
Deutschland