Differential-Display Reverse Transcription-P.C.R. (D.D.R.T.-P.C.R.) (Springer Lab Manuals) - Softcover

Colonna-Romano, Sergio

 
9783540632979: Differential-Display Reverse Transcription-P.C.R. (D.D.R.T.-P.C.R.) (Springer Lab Manuals)
Softcover
ISBN 10: 3540632972 ISBN 13: 9783540632979
Verlag: Springer-Verlag, 1998

Inhaltsangabe

Identification of differentially expressed genes is one of the major challenges in molecular biology. Several techniques allow the cloning of such sequences. However, methods such as RNA subtraction or differential hybridization are time-consuming and require large amounts of mRNA.
Recently, a new approach has successfully been developed: Differential-Display Reverse Transcription-PCR (DDRT-PCR). This technique has been proven to be highly effective in identifying sequences that are differentially expressed in various cell types. The most striking advantage is, however, that only nanograms of total RNA are sufficient. Thus every mRNA species expressed in the cell system can be investigated, even those present at very low levels.

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Identification of differentially expressed genes is one of the major challenges in molecular biology. Several techniques allow the cloning of such sequences. However, methods such as RNA subtraction or differential hybridization are time-consuming and require large amounts of mRNA.
Recently, a new approach has successfully been developed: Differential-Display Reverse Transcription-PCR (DDRT-PCR). This technique has been proven to be highly effective in identifying sequences that are differentially expressed in various cell types. The most striking advantage is, however, that only nanograms of total RNA are sufficient. Thus every mRNA species expressed in the cell system can be investigated, even those present at very low levels.

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Verlag
Springer-Verlag
Erscheinungsdatum
1998
Sprache
Englisch
ISBN 10 
3540632972
ISBN 13 
9783540632979
Einband
Tapa blanda
Anzahl der Seiten
136
Kontakt zum Hersteller
Nicht verfügbar
Verantwortliche Person
CMN Printpool Inh. Mathis Neumann
https://www.also.com/ec/cms5/en_6000/6000/index.jsp

Friedrich-Karl-Str. 9
Hamburg
Deutschland

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ISBN 10:  3642804551 ISBN 13:  9783642804557
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Softcover