Fullerene Derivatives: And Their Application in Bioanalytics - Softcover

Vallant, Rainer

Softcover

ISBN 10: 3639005406 ISBN 13: 9783639005400

Verlag: VDM Verlag Dr. Müller, 2008

Inhaltsangabe

Fullerene derivatives, such as dioctadecyl methano fullerene, fullerenoacetic acid, and IDA-[60]-fullerene were prepared and subjected to a comprehensive characterization study including protein binding properties and capacity. These fullerene derivatives were successfully applied as material-enhanced laser desorption/ionization (MELDI) carrier materials. It is shown that diverse functionalities result in characteristic human serum peak patterns (m/z 2000-20 000) which vary in terms of signal intensity as well as number of detectable masses. In addition, the fullerene derivatives clearly provided differences in the low molecular weight mass region (m/z 1000-4000) after elution of the adsorbed serum constituents. Monolithic, high-resolution capillary columns, were employed for eluate separation and target spotting. Thus, serum compounds were successfully fractionated and subjected to MS/MS analysis. This contribution, hence, proposes a new 'top-down' strategy for proteome research enabling protein profiling as well as biomarker identification in the low-mass range using selective enrichment, high-resolution separation, and offline MALDI-MS/MS evaluation.

Details zur Ausgabe

• Verlag: VDM Verlag Dr. Müller
• Erscheinungsdatum: 2008
• Sprache: Englisch
• ISBN 10: 3639005406
• ISBN 13: 9783639005400
• Einband: Taschenbuch
• Anzahl der Seiten: 84
• Kontakt zum Hersteller: Nicht verfügbar
• Verantwortliche Person: Buchpark GmbH; gpsr@buchpark.de; ; Krügerweg 1; Trebbin; Brandenburg; 14959; Deutschland