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AN OPTIMIZED EXPRESSION OF RECOMBINANT HEPCIDIN: USING PICHIA PASTORIS - Softcover

Rashid, Farzana; Ijaz Ali, Dr

 
9783639283242: AN OPTIMIZED EXPRESSION OF RECOMBINANT HEPCIDIN: USING PICHIA PASTORIS
Softcover
ISBN 10: 3639283244 ISBN 13: 9783639283242
Verlag: VDM Verlag Dr. Müller, 2010

Inhaltsangabe

Expression and purification of small peptides have always been problematic due to enzymatic degradation and many other technical problems. We report cloning and expression of a low molecular weight human antimicrobial peptide ?hepcidin' (Hepc, 20 amino acids) in pPIC9K transformed into P. pastoris GS115. The study reveals that active hepcidin peptide can be successfully expressed in this methylotrophic yeast. The BMMY medium was found to be optimal for the hepcidin protein expression and growth of the recombinant strains. Hepcidin protein expressed in recombinant strains was about 3 mg/L. Peptide expression was verified by Western blotting and ELISA assay. Recombinant hepc 20 was purified through Reverse-Phase HPLC column and characterized by Mass Spectrometry and amino acid sequencing. It also exhibited antibacterial activity against Staphylococcus aureus and Bacillus subtilis.

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Über die Autorinnen und Autoren

Dr Farzana Rashid is Assistant Professor at the Lahore University. She has 14 research publications, nine international and four national on her credit in the field of biotechnology, microbiology and education.Dr. Farzana has experience in coordinating Teacher Education programs and her interest areas include education and research.

Dr Farzana Rashid is Assistant Professor at the Lahore University. She has 14 research publications, nine international and four national on her credit in the field of biotechnology, microbiology and education.Dr. Farzana has experience in coordinating Teacher Education programs and her interest areas include education and research.

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Verlag
VDM Verlag Dr. Müller
Erscheinungsdatum
2010
Sprache
Englisch
ISBN 10 
3639283244
ISBN 13 
9783639283242
Einband
Taschenbuch
Anzahl der Seiten
136
Kontakt zum Hersteller
Nicht verfügbar
Verantwortliche Person
SIA OmniScriptum Publishing
customerservice@vdm-vsg.de

Brivibas gatve 197
Riga
1039
Lettland