Verwandte Artikel zu Screening for Reverse Cholesterol Transport efficiency

Screening for Reverse Cholesterol Transport efficiency - Softcover

Sengupta, Bhaswati

 
9783639664034: Screening for Reverse Cholesterol Transport efficiency
Softcover
ISBN 10: 3639664035 ISBN 13: 9783639664034
Verlag: Scholars' Press, 2014

Inhaltsangabe

Generation of foam cells, an essential step for reverse cholesterol transport (RCT) studies, uses the technique of receptor dependent macrophage loading with radiolabeled acetylated Low Density Lipoprotein (Ac-LDL). In this study, we used the ability of a biologically relevant detergent molecule, Lysophosphatidylcholine (Lyso PtdCho), to form mixed micelles with cholesterol or cholesteryl ester (CE) to generate macrophage foam cells. Fluorescent or radiolabelled cholesterol / Lyso PtdCho mixed micelles were prepared and incubated with RAW 264.7 or mouse peritoneal macrophages. Results showed that such micelles were quite stable at 4°C and retained the solubilized cholesterol during one month storage. Macrophages incubated with cholesterol or CE (unlabeled, fluorescently labeled or radiolabeled) / Lyso PtdCho mixed micelles accumulated CE as documented by microscopy, lipid staining, labeled oleate incorporation, and by thin layer chromatography (TLC). Such foam cells unloaded cholesterol when incubated with high density lipoprotein (HDL) and not with oxidized HDL (Ox-HDL). We propose that stable cholesterol or CE / Lyso PtdCho micelles would offer advantages over existing methods. O

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Über die Autorin bzw. den Autor

Bhaswati Sengupta is an Assistant Professor of Economics at Hofstra University in Long Island, NY. Her research focuses on labor, health and immigration issues and the application of Real Options theory in analyzing a variety of household decisions. She lives in Queens, New York with her husband, Mark.

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Generation of foam cells, an essential step for reverse cholesterol transport (RCT) studies, uses the technique of receptor dependent macrophage loading with radiolabeled acetylated Low Density Lipoprotein (Ac-LDL). In this study, we used the ability of a biologically relevant detergent molecule, Lysophosphatidylcholine (Lyso PtdCho), to form mixed micelles with cholesterol or cholesteryl ester (CE) to generate macrophage foam cells. Fluorescent or radiolabelled cholesterol / Lyso PtdCho mixed micelles were prepared and incubated with RAW 264.7 or mouse peritoneal macrophages. Results showed that such micelles were quite stable at 4°C and retained the solubilized cholesterol during one month storage. Macrophages incubated with cholesterol or CE (unlabeled, fluorescently labeled or radiolabeled) / Lyso PtdCho mixed micelles accumulated CE as documented by microscopy, lipid staining, labeled oleate incorporation, and by thin layer chromatography (TLC). Such foam cells unloaded cholesterol when incubated with high density lipoprotein (HDL) and not with oxidized HDL (Ox-HDL). We propose that stable cholesterol or CE / Lyso PtdCho micelles would offer advantages over existing methods. O

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Verlag
Scholars' Press
Erscheinungsdatum
2014
Sprache
Englisch
ISBN 10 
3639664035
ISBN 13 
9783639664034
Einband
Taschenbuch
Anzahl der Seiten
96
Kontakt zum Hersteller
Nicht verfügbar
Verantwortliche Person
Fromm
customerservice@vdm-vsg.de

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