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Quantitative DNA method in forensic DNA laboratory: Screening tool for efficient genotyping - Softcover
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Quantification of human DNA in forensic samples is an important step during STR profiling because the STR genotyping is sensitive to the quantity of DNA used in the PCR reaction.This study focuses on the importance of quantification in the entire process of genetic analysis.Two real time PCR platforms were used to compare two commercial kits in terms of DNA quantification.It was found out that accuracy of absolute quantification values in commercial quantification kits is strongly dependent on the construction of calibration curve. Especially low template DNA samples were used to assess whether QuantifilerTM or Plexor® HY System can determinate a minimum quantification value(cut off value)below which STR profiles would consistently fail to be detected. The usage of Plexor® HY System enabled to determine the cut off quantification value more exactly probably due to different molecular background and chemistry used in this kit.Reliability and other issues connected with cut off value are discussed. In order to better understand the relationship between the quantity of DNA and the number of detectable loci series the dilution expexperiment with standard DNA007 was done.
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Über die Autorin bzw. den Autor
Ivana Koljensic graduated from Charles University in Prague with a specialization anthropology and human genetics. During the study have focused on forensic genetics. She is a member of the international student organizations. And sees her future in forensic sciences.
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Quantitative DNA method in forensic DNA laboratory
Verlag:
LAP LAMBERT Academic Publishing Dez 2013, 2013
ISBN 10: 3659415804
ISBN 13: 9783659415807
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Taschenbuch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Quantification of human DNA in forensic samples is an important step during STR profiling because the STR genotyping is sensitive to the quantity of DNA used in the PCR reaction.This study focuses on the importance of quantification in the entire process of genetic analysis.Two real time PCR platforms were used to compare two commercial kits in terms of DNA quantification.It was found out that accuracy of absolute quantification values in commercial quantification kits is strongly dependent on the construction of calibration curve. Especially low template DNA samples were used to assess whether QuantifilerTM or Plexor® HY System can determinate a minimum quantification value(cut off value)below which STR profiles would consistently fail to be detected. The usage of Plexor® HY System enabled to determine the cut off quantification value more exactly probably due to different molecular background and chemistry used in this kit.Reliability and other issues connected with cut off value are discussed. In order to better understand the relationship between the quantity of DNA and the number of detectable loci series the dilution expexperiment with standard DNA007 was done. 88 pp. Englisch. Bestandsnummer des Verkäufers 9783659415807
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Quantitative DNA method in forensic DNA laboratory: Screening tool for efficient genotyping
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ISBN 13: 9783659415807
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Quantitative DNA Method in Forensic DNA Laboratory
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Quantitative DNA method in forensic DNA laboratory: Screening tool for efficient genotyping
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LAP LAMBERT Academic Publishing, 2013
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Zustand: Good. Your purchase helps support Sri Lankan Children's Charity 'The Rainbow Centre'. Ex-library, so some stamps and wear, but in good overall condition. Our donations to The Rainbow Centre have helped provide an education and a safe haven to hundreds of children who live in appalling conditions. Bestandsnummer des Verkäufers Z1-H-010-02406
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Quantitative DNA Method in Forensic DNA Laboratory
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Quantitative DNA Method in Forensic DNA Laboratory
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Quantitative DNA method in forensic DNA laboratory
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Zustand: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Autor/Autorin: Koljensic IvanaIvana Koljensic graduated from Charles University in Prague with a specialization anthropology and human genetics. During the study have focused on forensic genetics. She is a member of the international student organi. Bestandsnummer des Verkäufers 5154846
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Quantitative DNA method in forensic DNA laboratory
Verlag:
LAP LAMBERT Academic Publishing Dez 2013, 2013
ISBN 10: 3659415804
ISBN 13: 9783659415807
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Taschenbuch. Zustand: Neu. This item is printed on demand - Print on Demand Titel. Neuware -Quantification of human DNA in forensic samples is an important step during STR profiling because the STR genotyping is sensitive to the quantity of DNA used in the PCR reaction.This study focuses on the importance of quantification in the entire process of genetic analysis.Two real time PCR platforms were used to compare two commercial kits in terms of DNA quantification.It was found out that accuracy of absolute quantification values in commercial quantification kits is strongly dependent on the construction of calibration curve. Especially low template DNA samples were used to assess whether QuantifilerTM or Plexor® HY System can determinate a minimum quantification value(cut off value)below which STR profiles would consistently fail to be detected. The usage of Plexor® HY System enabled to determine the cut off quantification value more exactly probably due to different molecular background and chemistry used in this kit.Reliability and other issues connected with cut off value are discussed. In order to better understand the relationship between the quantity of DNA and the number of detectable loci series the dilution expexperiment with standard DNA007 was done.VDM Verlag, Dudweiler Landstraße 99, 66123 Saarbrücken 88 pp. Englisch. Bestandsnummer des Verkäufers 9783659415807
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Quantitative DNA method in forensic DNA laboratory : Screening tool for efficient genotyping
Verlag:
LAP LAMBERT Academic Publishing, 2013
ISBN 10: 3659415804
ISBN 13: 9783659415807
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Taschenbuch. Zustand: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - Quantification of human DNA in forensic samples is an important step during STR profiling because the STR genotyping is sensitive to the quantity of DNA used in the PCR reaction.This study focuses on the importance of quantification in the entire process of genetic analysis.Two real time PCR platforms were used to compare two commercial kits in terms of DNA quantification.It was found out that accuracy of absolute quantification values in commercial quantification kits is strongly dependent on the construction of calibration curve. Especially low template DNA samples were used to assess whether QuantifilerTM or Plexor® HY System can determinate a minimum quantification value(cut off value)below which STR profiles would consistently fail to be detected. The usage of Plexor® HY System enabled to determine the cut off quantification value more exactly probably due to different molecular background and chemistry used in this kit.Reliability and other issues connected with cut off value are discussed. In order to better understand the relationship between the quantity of DNA and the number of detectable loci series the dilution expexperiment with standard DNA007 was done. Bestandsnummer des Verkäufers 9783659415807
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Quantitative DNA method in forensic DNA laboratory | Screening tool for efficient genotyping
Verlag:
LAP LAMBERT Academic Publishing, 2014
ISBN 10: 3659415804
ISBN 13: 9783659415807
Neu
Taschenbuch
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Taschenbuch. Zustand: Neu. Quantitative DNA method in forensic DNA laboratory | Screening tool for efficient genotyping | Ivana Koljensic | Taschenbuch | 88 S. | Englisch | 2014 | LAP LAMBERT Academic Publishing | EAN 9783659415807 | Verantwortliche Person für die EU: preigu GmbH & Co. KG, Lengericher Landstr. 19, 49078 Osnabrück, mail[at]preigu[dot]de | Anbieter: preigu Print on Demand. Bestandsnummer des Verkäufers 105527090
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