Verwandte Artikel zu Transformation of Cotton with siRNA based Construct...

Transformation of Cotton with siRNA based Construct Targeting CLCuD: Transformation of Cotton (Gossypium hirsutum) with Amplicon Based Small Interfering RNA Targeting CLCuD - Softcover

Yasmeen, Aneela; Kiani, Sarfraz; Husnain, Tayyab

 
9783659698514: Transformation of Cotton with siRNA based Construct Targeting CLCuD: Transformation of Cotton (Gossypium hirsutum) with Amplicon Based Small Interfering RNA Targeting CLCuD
Softcover
ISBN 10: 3659698512 ISBN 13: 9783659698514
Verlag: LAP LAMBERT Academic Publishing, 2015

Inhaltsangabe

Cotton is an important crop. During past few years, cotton leaf curl disease (CLCuD) has emerged as an endemic. It has provided huge hindrance in production of high cotton yields. This disease is caused by cluster of begomovirus namely Cotton Leaf Curl Disease (CLCuD). Several breeding and transgenic approaches have been tried to overcome the problem. Among these RNA interference (RNAi) proved to be more promising. Use of RNA interference is favorable as the technique is environment friendly. In current research work RNAi based gene construct was transformed in two elite cotton verities MNH-786 and VH-289 to enhance viral resistance against CLCuD. The transformation of construct in plants was done by Agrobacterium mediated shoot apex method. The successful transformation and integration of the amplicon based RNAi construct was confirmed through PCR in T1 and T2 generation by using gene specific primers. Knock down ability of C2 construct was confirmed through real time CLCuD titer PCR. Comparison of virus disease index and Virus titer assured that the plants showing positive RNAi gene integration with minimum symptoms also had low viral titer.

Die Inhaltsangabe kann sich auf eine andere Ausgabe dieses Titels beziehen.

Über die Autorin bzw. den Autor

Aneela Yasmeen M. Phil: Molecular Biology RO cum Lecturer: Center of Excellence in Molecular Biology (CEMB), University of the Punjab, Lahore. Executive Member: National Academy of Young Scientists (NAYS), Pakistan Founding Member: Islamic World Young Scientists Academy (IYSA )Research Interests: Molecular biology; Plant Transformation.

„Über diesen Titel“ kann sich auf eine andere Ausgabe dieses Titels beziehen.

Verlag
LAP LAMBERT Academic Publishing
Erscheinungsdatum
2015
Sprache
Englisch
ISBN 10 
3659698512
ISBN 13 
9783659698514
Einband
Taschenbuch
Anzahl der Seiten
184
Kontakt zum Hersteller
Nicht verfügbar
Verantwortliche Person
Fromm
customerservice@vdm-vsg.de

Brivibas gatve 197
Riga
1039
Lettland