Development of a new microparticle vaccine adjuvant, MIS416 - Softcover

Mainini, Francesco

 
9783659946455: Development of a new microparticle vaccine adjuvant, MIS416
Softcover
ISBN 10: 3659946451 ISBN 13: 9783659946455
Verlag: LAP LAMBERT Academic Publishing, 2016

Inhaltsangabe

MIS416 is a novel vaccine adjuvant generated from propionibacterium acnes cell wall skeletons comprising naturally occurring ligands with the ability to induce effective adaptive immunity. In this research, I aimed to enhance the therapeutic potential of MIS416-adjuvanted vaccines by directly conjugating specific peptides derived from Tumors Associated Antigens (TAA) and siRNAs, achieving co-delivery of antigen and adjuvant. Using the high-affintiy streptavidin-biotin complex as a conjugation strategy for peptide attachment, we were able to irreversibly couple different fluorescent molecules to the surface of MIS416 as proof of principle. The OVA-derived peptide SIINFEKL, was then coupled to MIS416 and this MIS416-peptide conjugate was pulsed onto Dendritic cells (DC) showing that DC were activated by the conjugate. Flow cytometry analysis showed that levels of MHCII, CD40, CD80 and CD86 were upregulated by our novel vaccine conjugate. Proliferations assays have been carried out to prove that DC pulsed with MIS416-SIINFEKL are able to activate OT1 T cells and upregulate activation markers such as CD69.

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Reseña del editor

MIS416 is a novel vaccine adjuvant generated from propionibacterium acnes cell wall skeletons comprising naturally occurring ligands with the ability to induce effective adaptive immunity. In this research, I aimed to enhance the therapeutic potential of MIS416-adjuvanted vaccines by directly conjugating specific peptides derived from Tumors Associated Antigens (TAA) and siRNAs, achieving co-delivery of antigen and adjuvant. Using the high-affintiy streptavidin-biotin complex as a conjugation strategy for peptide attachment, we were able to irreversibly couple different fluorescent molecules to the surface of MIS416 as proof of principle. The OVA-derived peptide SIINFEKL, was then coupled to MIS416 and this MIS416-peptide conjugate was pulsed onto Dendritic cells (DC) showing that DC were activated by the conjugate. Flow cytometry analysis showed that levels of MHCII, CD40, CD80 and CD86 were upregulated by our novel vaccine conjugate. Proliferations assays have been carried out to prove that DC pulsed with MIS416-SIINFEKL are able to activate OT1 T cells and upregulate activation markers such as CD69.

Biografía del autor

Dr. Mainini Francesco earned his Bsc and Msc in Molecular Biotecnology from the University of Turin, Italy, in 2005 and 2007 respectively.He has obtained his PhD in Pathology in May 2016 from the University of Otago, New Zealand.

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Verlag
LAP LAMBERT Academic Publishing
Erscheinungsdatum
2016
Sprache
Englisch
ISBN 10 
3659946451
ISBN 13 
9783659946455
Einband
Tapa blanda
Anzahl der Seiten
244
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