Analysis of global gene expression profiles and invasion related genes of colorectal liver metastasis: Dissertationsschrift - Softcover

Bandapalli, Obul Reddy

Softcover

ISBN 10: 3741896608 ISBN 13: 9783741896606

Verlag: epubli, 2017

Inhaltsangabe

Liver is most frequently populated by metastases and may therefore serve as a model organ for studying metastatic invasion. For this reason, it was the aim of this thesis to understand the gene expression profiles and identify metastasis and invasion related genes in liver metastasis model. Differential gene expression was examined in three systems: A syngeneic mouse model (CT26/Balb/C mouse), a xenograft model (LS174T/nude mouse) and five clinical specimens. Gene expression profiles of a syngeneic mouse model and human clinical specimen revealed that the invasion front should be considered as a whole to find more overlapping potential target genes. Global gene expression studies on the host part of the invasion front revealed a pronounced overexpression of hepatic stellate cell activation markers at single gene level in this region demonstrating the feasibility of a differential gene expression approach on a genome wide scale. Global gene expression studies focusing on the tumor cells in vitro, in vivo and tumor part of the invasion front revealed an overall increase of cellular specialization from in vitro to invasion front. In addition to the secreted angiogeneic cytokines, β-catenin gene was elevated 9.6 fold in invasion front compared to in vitro. Evaluation of transcriptional up-regulation of β-catenin by promoter activity showed an 18.4 fold increase in the tumor cells of the invasion front as compared to the tumor cells in the inner parts of the tumor indicating a transcriptional mechanism of β-catenin regulation in addition to the well described post-translational regulatory mechanisms. In summary, application of high throughput Oligonucleotide microarray analysis in combination with real time PCR technology allowed the identification of genes, which might play a role in proliferation, invasion and angiogenesis of tumors in colorectal liver metastasis.

Über diesen Titel

Über die Autorin bzw. den Autor

PD. Dr.rer.nat. Obul R Bandapalli is a functional genomics researcher with special focus on transnational research. He is currently working at DKFZ, Heidelberg, Germany. He finished his habilitation in Molecular Medicine from the Molecular Medicine Partnership Unit of EMBL-University of Heidelberg in and continues to teach at the University of Heidelberg. He has PhD in Molecular Biology from Humboldt University. Before moving to Germany he has worked at the Central Food Technological Research Institute Mysore, Dabur Research Foundation and Jawaharlal Nehru University, New Delhi, India. He obtained his Master´s in Biotechnology from Guru Ghasidas University and Bachelor’s in Sericulture from Sri Venkateswara University. Apart from research, he enjoys watching birds and nature trails.

Details zur Ausgabe

• Verlag: epubli
• Erscheinungsdatum: 2017
• Sprache: Englisch
• ISBN 10: 3741896608
• ISBN 13: 9783741896606
• Einband: Taschenbuch
• Auflage: 1
• Anzahl der Seiten: 124
• Kontakt zum Hersteller: Neopubli GmbH; kontakt@epubli.de; www.epubli.de; ; Köpenicker Str. 154a; Berlin; 10997; Deutschland