Microparticle Formation by Spray-Freeze-Drying: The Influence of Atomization Conditions on Protein Secondary and Tertiary Structure - Softcover

Vonhoff, Sebastian

 
9783838117713: Microparticle Formation by Spray-Freeze-Drying: The Influence of Atomization Conditions on Protein Secondary and Tertiary Structure
Softcover
ISBN 10: 3838117719 ISBN 13: 9783838117713
Verlag: Suedwestdeutscher Verlag fuer Hochschulschriften, 2010

Inhaltsangabe

This thesis deals with the preparation microparticles by spray-freeze-drying (SFD). As the process consists of various unit operations, the stability of labile APIs, such as proteins, can be serverly impaired. The secondary structure of a protein is known to be directly linked to its overall stability. However, quantification of protein secondary structure by FTIR spectroscopy can show poor reproducibility. Therefore, the first part of this thesis covers the development of a new FTIR method for objective and fast determination of protein secondary structure. The second part investigates the influence of atomization conditions on protein secondary and tertiary structure as well as residual enzyme activity during SFD. The process is analyzed after a) atomization b) atomization, freezing and thawing and c) the complete SFD process. A thorough comparison of the utilized ultrasound nozzles (including particle size distribution, temperatures and cavitation effects) is performed in the third part of this thesis. In the fourth and last part, different formulations containing a mixture of alpha-chymotrypsin and various excipients are evaluated for their stability.

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Über die Autorin bzw. den Autor

Sebastian Vonhoff passed his licensing exam as German pharmacistin 2006. From 2006 - 2009 he did his PhD thesis under thesupervision of Prof. Dr. Geoffrey Lee at the Division ofPharmaceutics (University Erlangen-Nuremberg). Focus laid onanalysis of protein secondary structure by FTIR spectroscopy andparticle formation by spray-freeze-drying.

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Verlag
Suedwestdeutscher Verlag fuer Hochschulschriften
Erscheinungsdatum
2010
Sprache
Englisch
ISBN 10 
3838117719
ISBN 13 
9783838117713
Einband
Taschenbuch
Anzahl der Seiten
188
Kontakt zum Hersteller
Nicht verfügbar
Verantwortliche Person
Presses Academiques Francophones
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Brivibas gatve 197
Riga
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