Enzyme technology using Bacterial lipid modification enzyme LGT: Characterization of LGT with a new generic peptide assay and LGT-bioreactor prototype for lipopeptide synthesis - Softcover

Sankaran, Krishnan

 
9783838323541: Enzyme technology using Bacterial lipid modification enzyme LGT: Characterization of LGT with a new generic peptide assay and LGT-bioreactor prototype for lipopeptide synthesis
Softcover
ISBN 10: 3838323548 ISBN 13: 9783838323541
Verlag: LAP Lambert Academic Publishing, 2009

Inhaltsangabe

Covalent lipid modification of proteins anchors hydrophilic proteins/peptides to hydrophobic surfaces and aid efficient performance in aqueous conditions as required in many biotechnological applications like ELISA, biosensors, targeted-drug delivery and liposomal integration. Lipid modification studied by him in bacteria extended the possibility of in vitro lipid modification, particularly for peptides. In this thesis the first enzyme of the pathway, phosphatidylglycerol:prolipoprotein diacylglyceryl transferase (Lgt), which catalyzes the bulk of the lipidation has been utilized in devising a small bioreactor prototype for in vitro peptide modification. To achieve this, a new generic synthetic peptide assay was designed and used for its kinetic characterization and enrichment. The packed bed reactor with continuous and total feed-back operation in batch mode converted 65% of the model hydrophilic peptide substrate to lipopeptide. The higher conversion rate achieved in this study demonstrated the feasibility of such a bioreactor for lipid modification of a variety of peptides for potential biotech applications.

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Über die Autorin bzw. den Autor

A doctorate in Biochemistry from University of Hyderabad Prof. Sankaran joined USUHS,USA as PDF in 1990 and discovered the bacterial lipid modification pathway. From 1995 onwards at Anna University, he continues on its applications. He has authored 30 publications and 6 reviews. Dr. Tamil Selvan is a doctorate in Biotechnology from Anna University.

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Verlag
LAP Lambert Academic Publishing
Erscheinungsdatum
2009
Sprache
Englisch
ISBN 10 
3838323548
ISBN 13 
9783838323541
Einband
Taschenbuch
Anzahl der Seiten
208
Kontakt zum Hersteller
Nicht verfügbar
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