Comparative Study of IVF and parthenogenesis in bubaline oocytes: IVF and parthenogenesis: A comparative study - Softcover

Mishra, Vivek; K Misra, A

 
9783844326963: Comparative Study of IVF and parthenogenesis in bubaline oocytes: IVF and parthenogenesis: A comparative study
Softcover
ISBN 10: 3844326960 ISBN 13: 9783844326963
Verlag: LAP LAMBERT Academic Publishing, 2011

Inhaltsangabe

The objective of this study was to understand the pitfalls of the poor oocyte cleavage and subsequent embryo production by assessing the developmental competence of the oocytes in buffalo using parthenogenesis as a model which was compared with IVF (natural activation). Buffalo ovaries were collected from slaughter house and matured oocytes were subjected to either IVF (control) or chemical activation (treatment)[7% ethanol (ET)for 7 min + 2.5 mM 6-Di methyl amino purine (6-DMAP) for 4 hr, or 7% ET for 7 min + 10 ug/ml Cycloheximide (CHX) for 6 hr or 7% ET for 7 min + 2.5 mM 6-DMAP + 10 ug/ml CHX for 6 hr]. Fertilized and chemically activated oocytes were cultured in mSOF medium for 8 days to study embryo development.In conclusion, the results of parthenogenesis, revealed that buffalo oocytes had better inherent developmental competence and the poor cleavage and embryo development following IVF, in our study, may partly be due to the poor quality of frozen-thawed sperm, improper sperm capacitation and/ or fertilization. Further, high ambient temperature adversely affected the cleavage and embryo development following IVF.

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Reseña del editor

The objective of this study was to understand the pitfalls of the poor oocyte cleavage and subsequent embryo production by assessing the developmental competence of the oocytes in buffalo using parthenogenesis as a model which was compared with IVF (natural activation). Buffalo ovaries were collected from slaughter house and matured oocytes were subjected to either IVF (control) or chemical activation (treatment)[7% ethanol (ET)for 7 min + 2.5 mM 6-Di methyl amino purine (6-DMAP) for 4 hr, or 7% ET for 7 min + 10 ug/ml Cycloheximide (CHX) for 6 hr or 7% ET for 7 min + 2.5 mM 6-DMAP + 10 ug/ml CHX for 6 hr]. Fertilized and chemically activated oocytes were cultured in mSOF medium for 8 days to study embryo development.In conclusion, the results of parthenogenesis, revealed that buffalo oocytes had better inherent developmental competence and the poor cleavage and embryo development following IVF, in our study, may partly be due to the poor quality of frozen-thawed sperm, improper sperm capacitation and/ or fertilization. Further, high ambient temperature adversely affected the cleavage and embryo development following IVF.

Biografía del autor

The author of this book, Vivek Mishra born on 30/06/1983 in Gorakhpur, UP, India. He passed his Highschool, Intermediate, BSc and MSc (Mol Biol & Biotech) examination in 1997, 1999, 2003 & 2005, respectively with first division. The author has completed his PhD in Biochemistry and published several papers in international journal.

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Verlag
LAP LAMBERT Academic Publishing
Erscheinungsdatum
2011
Sprache
Englisch
ISBN 10 
3844326960
ISBN 13 
9783844326963
Einband
Tapa blanda
Anzahl der Seiten
140
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