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Methodology for Extraction and Purification of ISOMERASE: Polarimetric Determination of Mutarotase; an Isomerase - Softcover

Siddique, Amna; Khurshid, Shazia

 
9783846522530: Methodology for Extraction and Purification of ISOMERASE: Polarimetric Determination of Mutarotase; an Isomerase
Softcover
ISBN 10: 3846522538 ISBN 13: 9783846522530
Verlag: LAP LAMBERT Academic Publishing, 2011

Inhaltsangabe

Mutarotases are recently characterized group of enzymes that are involved in anomeric conversions of monosaccharides enhancing rate of metabolism. Mutarotase was extracted and purified from bovine kidney cortex by (NH4)2SO4 precipitation technique, dialysis and gel filtration chromatography. Polarimeter was used for assay of Mutarotase in this present project. Mutarotase increased activity from 0.478 to 1.711 U/ml by purification. It gained 13.4 folds purification at this final step. It is helpful in determining the glucose level and participates in glycolysis and gluconeogenesis. Activities and fold purification of Mutarotase can also be increased by much improved techniques of purification like ion exchange chromatography or FPLC hence enhancing the production of this enzyme.

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Über die Autorin bzw. den Autor

Amna Siddique: M.Phil Chemistry, GC University Lahore, Pakistan worked on Production of Enzymes and their Purification.

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Verlag
LAP LAMBERT Academic Publishing
Erscheinungsdatum
2011
Sprache
Englisch
ISBN 10 
3846522538
ISBN 13 
9783846522530
Einband
Taschenbuch
Anzahl der Seiten
72
Kontakt zum Hersteller
Nicht verfügbar
Verantwortliche Person
SIA OmniScriptum Publishing
customerservice@vdm-vsg.de

Brivibas gatve 197
Riga
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