Die Kultivierung filamentöser Pilze ist von großer wirtschaftlicher und wissenschaftlicher Bedeutung. Dennoch wurde der Zustand von Pilzsporen, die als Inokulum den Startpunkt der meisten Pilzkultivierungen darstellen, bislang vernachlässigt. In dieser Arbeit werden daher neun Qualitätsindikatoren für die Charakterisierung von Konidien vorgestellt. Diese ermöglichen, das Sporulationsenvironom, die Sporeneigenschaften sowie die Ergebnisse einer anschließenden Submerskultivierung zu korrelieren. Als Modellorganismus dient Aspergillus ochraceus, der exemplarische Produktionsprozess ist die Hydroxylierung eines Steroids. Zwischen dem Kohlenstoffgehalt des Sporulationsmediums und der Konidienausbeute wurde ein positiver Zusammenhang festgestellt. Die höchste Konzentration ergab Haferkleie-Agar mit Ausbeuten von 26 • 107 Sporen/mL. Des Weiteren wurden 126 Metabolite in Sporen detektiert, die größtenteils zur Gruppe der Polyole gehören und in Konzentrationen von bis zu 55 ng/105 Sporen vorliegen. Eine enge Verbindung zur Lagerungsfestigkeit und der Aktivität der Sporen konnte auch hier gezeigt werden. Der Eduktumsatz liegt nach 72 h maximal bei ca. 80 %. Weitere wichtige Einflussfaktoren für die Kulturausbeute sind der Gehalt an Protein gebundenem NAD(P)H sowie das Aggregationsverhalten, welches die Morphologie beeinflusst. Sehr starke Veränderungen der Sporen wurden bereits während der Lagerung in Suspension bei 4 °C für 7 Tage festgestellt.
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Taschenbuch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Cultivations of filamentous fungi are of large industrial and scientific interest. However, the utmost starting point of the majority of those cultivations, the inoculum consisting of fungal spores, has previously been neglected. This work hence introduces a total of nine spore quality indicators and subsequently correlates the sporulation environome to the properties of the generated spores and their performance in submerged cultivation. The model organism is Aspergillus ochraceus while the model process is the hydroxylation of a steroid. A positive correlation between the spore yield and the substrate¿s carbon content on solid medium was found with oat bran agar giving best yields of 26 107 spores/mL at carbon concentrations of 15.4 g/L in the solid medium. The assessment of the conidial metabolome revealed the presence of 126 metabolites. Particularly polyols, such as glycerol, erythritol and ribitol, are found at high concentrations of up to 55 ng/105 spores. It was further shown, that several of these metabolites are degraded within the spores during storage for 7 d in saline solution. Their concentrations also strongly correlate with the storage resistance of the conidia as well as with their performance in subsequent submerged cultivations. Highest reactant turnover of approx. 80 % was determined after 72 h of cultivation with spores generated on wheat bran agar. The culture performance is further a function of the portion of protein bound NADH, which was determined and spatially visualized by means of two-photon fluorescence lifetime imaging. 182 pp. Englisch. Bestandsnummer des Verkäufers 9783869558479
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Zustand: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. KlappentextrnrnCultivations of filamentous fungi are of large industrial and scientific interest. However, the utmost starting point of the majority of those cultivations, the inoculum consisting of fungal spores, has previously been neglected. . Bestandsnummer des Verkäufers 119413087
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Taschenbuch. Zustand: Neu. This item is printed on demand - Print on Demand Titel. Neuware -Cultivations of filamentous fungi are of large industrial and scientific interest. However, the utmost starting point of the majority of those cultivations, the inoculum consisting of fungal spores, has previously been neglected. This work hence introduces a total of nine spore quality indicators and subsequently correlates the sporulation environome to the properties of the generated spores and their performance in submerged cultivation. The model organism is Aspergillus ochraceus while the model process is the hydroxylation of a steroid. A positive correlation between the spore yield and the substrate's carbon content on solid medium was found with oat bran agar giving best yields of 26 107 spores/mL at carbon concentrations of 15.4 g/L in the solid medium. The assessment of the conidial metabolome revealed the presence of 126 metabolites. Particularly polyols, such as glycerol, erythritol and ribitol, are found at high concentrations of up to 55 ng/105 spores. It was further shown, that several of these metabolites are degraded within the spores during storage for 7 d in saline solution. Their concentrations also strongly correlate with the storage resistance of the conidia as well as with their performance in subsequent submerged cultivations. Highest reactant turnover of approx. 80 % was determined after 72 h of cultivation with spores generated on wheat bran agar. The culture performance is further a function of the portion of protein bound NADH, which was determined and spatially visualized by means of two-photon fluorescence lifetime imaging.Cuvillier Verlag, Nonnenstieg 8, 37075 Göttingen 182 pp. Englisch. Bestandsnummer des Verkäufers 9783869558479
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Taschenbuch. Zustand: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - Cultivations of filamentous fungi are of large industrial and scientific interest. However, the utmost starting point of the majority of those cultivations, the inoculum consisting of fungal spores, has previously been neglected. This work hence introduces a total of nine spore quality indicators and subsequently correlates the sporulation environome to the properties of the generated spores and their performance in submerged cultivation. The model organism is Aspergillus ochraceus while the model process is the hydroxylation of a steroid. A positive correlation between the spore yield and the substrate¿s carbon content on solid medium was found with oat bran agar giving best yields of 26 107 spores/mL at carbon concentrations of 15.4 g/L in the solid medium. The assessment of the conidial metabolome revealed the presence of 126 metabolites. Particularly polyols, such as glycerol, erythritol and ribitol, are found at high concentrations of up to 55 ng/105 spores. It was further shown, that several of these metabolites are degraded within the spores during storage for 7 d in saline solution. Their concentrations also strongly correlate with the storage resistance of the conidia as well as with their performance in subsequent submerged cultivations. Highest reactant turnover of approx. 80 % was determined after 72 h of cultivation with spores generated on wheat bran agar. The culture performance is further a function of the portion of protein bound NADH, which was determined and spatially visualized by means of two-photon fluorescence lifetime imaging. Bestandsnummer des Verkäufers 9783869558479
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Taschenbuch. Zustand: Neu. Development and Validation of Indicators for the Production and Quality of Seed Cultures | Matthias Gehder | Taschenbuch | Schriftenreihe des Institutes für Bioverfahrenstechnik der Technischen Universität Braunschweig 59 | Englisch | 2011 | Cuvillier | EAN 9783869558479 | Verantwortliche Person für die EU: Cuvillier Verlag, Nonnenstieg 8, 37075 Göttingen, info[at]cuvillier[dot]de | Anbieter: preigu Print on Demand. Bestandsnummer des Verkäufers 120685447
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