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NGS Approach for Variant Discovery in Giant Freshwater Prawn: An NGS approach to mine SNP from the amplicons of Macrobrachium rosenbergii - Softcover

Agarwal, Deepak; Chaudhari, Aparna

 
9786200440051: NGS Approach for Variant Discovery in Giant Freshwater Prawn: An NGS approach to mine SNP from the amplicons of Macrobrachium rosenbergii
Softcover
ISBN 10: 6200440050 ISBN 13: 9786200440051
Verlag: LAP LAMBERT Academic Publishing, 2019

Inhaltsangabe

Amongst farmed crustaceans, the giant freshwater prawn Macrobrachium rosenbergii was until recently one of the major species with high commercial value. Lately, it has lost popularity due to diseases and differential growth of males on account of social hierarchy. The species can reacquire its former preferred status if it is genetically improved for growth and disease resistance. Selective breeding is greatly facilitated by marker-assisted selection (MAS). Single nucleotide polymorphisms (SNPs) offer the finest resolution possible for a marker map (a single nucleotide change), are co-dominant, bi-allelic and generally abundant in populations with a low mutation rate. DNA sequencing through NGS platforms is regarded as the most cost-effective approach. In this study, SNP mining was carried out in the transcribed sequences of M. rosenbergii available in GenBank, NCBI. Wild specimens were collected from different water bodies. Primers were designed to amplify coding (CDS) and sequenced the amplicons on GS-FLX 454 and Ion torrent platforms. 173 SNPs were detected across populations. This approach for mining SNPs in reported sequences has been used by us for the first time.

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Über die Autorin bzw. den Autor

Deepak Agarwal has done graduation in fisheries science from G.B.P.U.A & T Pantnagar University. He holds his Ph.D. in Fish Biotechnology from CIFE, Mumbai. He received a VC gold medal for the highest marks in UG and the Best dissertation award for master's work. He is working now as an Assistant Professor.

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Verlag
LAP LAMBERT Academic Publishing
Erscheinungsdatum
2019
Sprache
Englisch
ISBN 10 
6200440050
ISBN 13 
9786200440051
Einband
Taschenbuch
Anzahl der Seiten
88
Kontakt zum Hersteller
Nicht verfügbar
Verantwortliche Person
SIA OmniScriptum Publishing
customerservice@vdm-vsg.de

Brivibas gatve 197
Riga
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Lettland