Expression and Characterization of Nucleocapsid (N) Gene of R.P.Virus - Softcover

Shrivastava, Neeraj; Sharma, Bhaskar; Shrivastav, Arpita

Softcover

ISBN 10: 6200587884 ISBN 13: 9786200587886

Verlag: LAP LAMBERT Academic Publishing, 2020

Inhaltsangabe

The book is MVSc research work of the author on recombinant plasmid containing N gene of rinderpest (pBK-CMV-39) of eukaryotic expression of N protein. The fusion protein region along with prokaryotic promoter was removed by Nhel-Spel digestion. The digested plasmid was religated and was used for transforming XL-1 Blue MRF’ cells From the transformed cells the plasmid (pBK-CMV-NS) was purified and characterized by RE digestion. Both the plasmids were used for eukaryotic expression studies.While the cells transfected with pBK-CMV-G9 show expression of a protein of the size 60 kD which corresponds to size of N protein of RPV. The expressed protein reacted with rinderpest polyclonal serum in western blot and ELISA. This indicate that the expressed protein resembles N protein of RPV in immunogenicity. Localization by FAT showed expressed protein was predominantly localized in the cytoplasm of transfected cells. The Number transfected cells were very low.

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Über die Autorin bzw. den Autor

Dr Neeraj Shrivastava (Ph.D) is working as Assistant Professor in the Department of Veterinary Microbiology at N.D.V.S.U.,Jabalpur(M.P.)The present work is his MVSc thesis.Dr Shrivastava is a gold medalist in B.V.Sc & A.H.He has various research articles in his credit.At present he is associated with antimicrobial resistance and MRSA.

Details zur Ausgabe

• Verlag: LAP LAMBERT Academic Publishing
• Erscheinungsdatum: 2020
• Sprache: Englisch
• ISBN 10: 6200587884
• ISBN 13: 9786200587886
• Einband: Taschenbuch
• Anzahl der Seiten: 64
• Kontakt zum Hersteller: Nicht verfügbar
• Verantwortliche Person: SIA OmniScriptum Publishing; customerservice@vdm-vsg.de; ; Brivibas gatve 197; Riga; 1039; Lettland