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Class I HLA in Normal Human Tissues: Laboratory Data.: Quantification by RT-PCR - Softcover

García-Ruano, Ana Belén; Martín-Alcolea, María Amparo; García-Chileme, Soledad

 
9786203718850: Class I HLA in Normal Human Tissues: Laboratory Data.: Quantification by RT-PCR
Softcover
ISBN 10: 6203718858 ISBN 13: 9786203718850
Verlag: Our Knowledge Publishing, 2021

Inhaltsangabe

To know if the transcription level of a certain gene (HLA-A, HLA-B or HLA-C) is altered in tumour cells, we should first know what the transcription level is in the normal cells from which these tumours derive.Therefore, in our study we will try to quantitatively measure the locus-specific gene expression of HLA-ABC in normal tissues. In order to perform this measurement we have designed primers that specifically amplify these locus by real-time PCR. The normal tissues chosen were peripheral blood leukocytes and colon and larynx mucosa. In order to select the appropriate cells, thus avoiding interferences, laser microdissection has been performed in both colon and larynx mucosa. Throughout this work we will provide evidence of the specificity of the technique as well as the patterns of gene expression found in the different tissues studied.

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Über die Autorin bzw. den Autor

Dit boek is geschreven door drie Area Medical Specialists van de Clinical Analysis Clinical Management Unit van het Universitair Ziekenhuis van Jaén.

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Verlag
Our Knowledge Publishing
Erscheinungsdatum
2021
Sprache
Englisch
ISBN 10 
6203718858
ISBN 13 
9786203718850
Einband
Taschenbuch
Anzahl der Seiten
52
Kontakt zum Hersteller
SIA OmniScriptum Publishing
customerservice@vdm-vsg.de

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Riga
1039
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