A Practical Guide to Influenza Virus Isolation and Identification - Softcover

Boora, Sanjit; Khan, Anish; Kaushik, Samander

Softcover

ISBN 10: 6209309305 ISBN 13: 9786209309304

Verlag: LAP LAMBERT Academic Publishing, 2025

Inhaltsangabe

This practical guide details the essential methodologies for the isolation, identification, and quantification of Influenza A viruses, specifically focusing on the H1N1pdm09 strain using MDCK cell culture systems. It provides a comprehensive workflow that includes standardized protocols for the maintenance and subculturing of MDCK cells, viral propagation techniques, and molecular confirmation via RT-PCR targeting the HA gene. Furthermore, the text offers in-depth instruction on viral quantification methods, employing Hemagglutination (HA) assays to determine total viral titers and TCID50 assays using the Reed-Muench method for calculating infectious titers. By combining theoretical background with rigorous experimental data and step-by-step procedural flowcharts, this work serves as a vital resource for researchers and laboratory professionals establishing robust platforms for influenza virus characterization and antiviral drug discovery.

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Über die Autorin bzw. den Autor

Sanjit Boora is a Senior Research Fellow at the Centre for Biotechnology, Maharshi Dayanand University (MDU), Rohtak. His research focuses on virology, with a specialization in the isolation and characterization of Influenza viruses, the development of rapid molecular diagnostic tools, and evaluation of the antiviral potential of medicinal plants.

Details zur Ausgabe

• Verlag: LAP LAMBERT Academic Publishing
• Erscheinungsdatum: 2025
• Sprache: Englisch
• ISBN 10: 6209309305
• ISBN 13: 9786209309304
• Einband: Taschenbuch
• Anzahl der Seiten: 52
• Kontakt zum Hersteller: preigu GmbH & Co. KG; mail@preigu.de; ; Lengericher Landstr. 19; Osnabrück; Niedersachsen; 49078; Deutschland