The proliferation of nanotechnologies, particularly in the food sector, raises concerns about their safety for human health. Due to their nanometric size, these particles cross biological barriers, from the mouth to the intestine. This work assesses the toxicological impact of two nanoparticle (NP) mixtures: TiO₂/Ag and SiO₂/Ag on Escherichia coli, a bacterium representative of the intestinal flora. A clinical strain was isolated by coproculture, identified (API 20E), then exposed to dilutions of NPs (0.125-1024 μg/ml). Petri dishes in Hecktoen medium revealed phenotypic alterations after 24 h incubation at 37°C: decreased growth, reduced colonies, morphological changes, loss of mobility and variations in grouping. At certain concentrations (2 μg/mL TiO₂/Ag, 4 μg/mL SiO₂/Ag), genomic changes were observed (Gram staining), suggesting a potential imbalance in the gut microbiota. These results highlight the need for further study by molecular analysis (DNA extraction, sequencing) to better understand NPs-induced mutations.
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Dr Amina Bouzana, badaczka w dziedzinie mikrobiologii stosowanej i absolwentka Uniwersytetu Skikda (Algieria), interesuje si¿ powi¿zaniami mi¿dzy mikrobiologi¿ a zdrowiem publicznym. Jej badania maj¿ na celu zrozumienie wp¿ywu zagröe¿ mikrobiologicznych na zdrowie publiczne i opracowanie nowych podej¿¿ prewencyjnych.
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Taschenbuch. Zustand: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - The proliferation of nanotechnologies, particularly in the food sector, raises concerns about their safety for human health. Due to their nanometric size, these particles cross biological barriers, from the mouth to the intestine. This work assesses the toxicological impact of two nanoparticle (NP) mixtures: TiO2/Ag and SiO2/Ag on Escherichia coli, a bacterium representative of the intestinal flora. A clinical strain was isolated by coproculture, identified (API 20E), then exposed to dilutions of NPs (0.125-1024 mig/ml). Petri dishes in Hecktoen medium revealed phenotypic alterations after 24 h incubation at 37°C: decreased growth, reduced colonies, morphological changes, loss of mobility and variations in grouping. At certain concentrations (2 mig/mL TiO2/Ag, 4 mig/mL SiO2/Ag), genomic changes were observed (Gram staining), suggesting a potential imbalance in the gut microbiota. These results highlight the need for further study by molecular analysis (DNA extraction, sequencing) to better understand NPs-induced mutations. Bestandsnummer des Verkäufers 9786209334108
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Paperback. Zustand: new. Paperback. The proliferation of nanotechnologies, particularly in the food sector, raises concerns about their safety for human health. Due to their nanometric size, these particles cross biological barriers, from the mouth to the intestine. This work assesses the toxicological impact of two nanoparticle (NP) mixtures: TiO2/Ag and SiO2/Ag on Escherichia coli, a bacterium representative of the intestinal flora. A clinical strain was isolated by coproculture, identified (API 20E), then exposed to dilutions of NPs (0.125-1024 mg/ml). Petri dishes in Hecktoen medium revealed phenotypic alterations after 24 h incubation at 370C: decreased growth, reduced colonies, morphological changes, loss of mobility and variations in grouping. At certain concentrations (2 mg/mL TiO2/Ag, 4 mg/mL SiO2/Ag), genomic changes were observed (Gram staining), suggesting a potential imbalance in the gut microbiota. These results highlight the need for further study by molecular analysis (DNA extraction, sequencing) to better understand NPs-induced mutations. This item is printed on demand. Shipping may be from our UK warehouse or from our Australian or US warehouses, depending on stock availability. Bestandsnummer des Verkäufers 9786209334108
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Taschenbuch. Zustand: Neu. This item is printed on demand - Print on Demand Titel. Neuware -The proliferation of nanotechnologies, particularly in the food sector, raises concerns about their safety for human health. Due to their nanometric size, these particles cross biological barriers, from the mouth to the intestine. This work assesses the toxicological impact of two nanoparticle (NP) mixtures: TiO¿/Ag and SiO¿/Ag on Escherichia coli, a bacterium representative of the intestinal flora. A clinical strain was isolated by coproculture, identified (API 20E), then exposed to dilutions of NPs (0.125-1024 ¿g/ml). Petri dishes in Hecktoen medium revealed phenotypic alterations after 24 h incubation at 37°C: decreased growth, reduced colonies, morphological changes, loss of mobility and variations in grouping. At certain concentrations (2 ¿g/mL TiO¿/Ag, 4 ¿g/mL SiO¿/Ag), genomic changes were observed (Gram staining), suggesting a potential imbalance in the gut microbiota. These results highlight the need for further study by molecular analysis (DNA extraction, sequencing) to better understand NPs-induced mutations.VDM Verlag, Dudweiler Landstraße 99, 66123 Saarbrücken 76 pp. Englisch. Bestandsnummer des Verkäufers 9786209334108
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