HPLC-UV Method development for Fixed Dose Pharmaceutical Formulations: Analytical Method Development and Validation for selected Fixed-Dose Formulations Using HPLC and UV Spectroscopy - Softcover

Yadav, Sonia; Sharma, Anunny; Maratha, Sushma

 
9786209779718: HPLC-UV Method development for Fixed Dose Pharmaceutical Formulations: Analytical Method Development and Validation for selected Fixed-Dose Formulations Using HPLC and UV Spectroscopy
Softcover
ISBN 10: 6209779719 ISBN 13: 9786209779718
Verlag: LAP LAMBERT Academic Publishing, 2026

Inhaltsangabe

Estimation of imeglimin hydrochloride in human plasma by HPLC-UV. The estimation of drugs by RP-HPLC (reverse phase chromatography) was chosen since it is advised for use with ionic and mild to non-polar compounds. Simple, specific, and superior in terms of efficiency, stability, and reproducibility is reverse phase chromatography. For the separation of imeglimin, the C18 column 250 x 4.6 mm, 5 µm particle size was chosen. In the mobile phase, various solvent solutions were tested and combined for optimum performance. Imeglimin hydrochloride (4 µg/ml) in buffer, pH 4 for Acetonitrile: Water (70:30), a concentration range (8-40 and 0.5-4 µg/ml) displaying excellent peak and a sizable resolution level. Having the mobile phase run at 1ml/min, photodiode array detectors were used to detect both analytes at 215 nm. The method was validated in different concentration ranges in human plasma. Imeglimin inter and intra-run precision was measured at less than 3.60% and had an accuracy of less than 1.869%. imeglimin in human plasma was validated by linearity, recovery matrix, and stability. The retention time of IMEG is 4.8 minutes in plasma. Suggested method denotes that it is cost-effective

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Über die Autorin bzw. den Autor

Dr. Sonia Yadav is an Associate Professor of Pharmaceutical Sciences with over 15 years of teaching experience. She has been associated with SGT University since 2015 and specializes in Pharmaceutical Analysis. Her interests include pharmacy education, analytical techniques, and mentoring pharmacy students for academic and professional excellence.

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Verlag
LAP LAMBERT Academic Publishing
Erscheinungsdatum
2026
Sprache
Englisch
ISBN 10 
6209779719
ISBN 13 
9786209779718
Einband
Taschenbuch
Anzahl der Seiten
52
Kontakt zum Hersteller
Edicoes Nosso Conhecimento
customerservice@vdm-vsg.de

SIA
Riga
1039
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