The seven sections of this book cover general laboratory procedures, such as pipetting, solution preparation, and the operation of common analytical instruments, as well as specific techniques for working with nucleic acids, bacteria, enzymes, and proteins. The book also includes information on cloning experiments and plant biotechnology protocols. The text emphasizes the isolation of DNA and RNA from various sources, the use of restriction enzymes, ligation techniques, cloning protocols, and the screening of transformed cells. Additionally, it covers a range of electrophoresis techniques, PCR protocols, and other essential laboratory techniques.The appendices at the end of the book provide supplementary information that is relevant to the study of the practical applications covered throughout the manual. This book will be of particular use to students in the fields of biotechnology, agriculture, and related disciplines. The goal of this practical manual is to provide a clear and comprehensive theoretical foundation for the practical laboratory studies that students will undertake.
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Sunita Dutta: Reader, Department of Zoology, Women's College, Ranchi,Jharkhand, India.
Abhijit Dutta: Reader, Department of Zoology,University of Ranchi, Ranchi, Jharkhand, India.
Ashok Kumar Choudhary: Professor, Department of Botany University of Ranchi, Ranchi, Jharkhand, India.
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Hardcover. Zustand: As New. tables, figs (illustrator). Contents Preface Abbreviation 1 General procedures 2 Nucleic acids and Ecoli 3 Enzymes 4 Cloning 5 DNARNA separation 6 Proteins 7 Plant biotechnology Appendices Glossary References IndexThe book is subdivided into seven sections this encompass general procedures like methods of pipetting solution preparation buffers and principles of common analytical instruments essential for laboratory biotechnology experimentsThe book also includes working with nucleic acid bacteria enzymes proteins; cloning experiments and a few protocols on plant biotechnology Emphasis have been given on DNARNA isolation from various sources use of restriction enzymes ligation techniques cloning protocols screening of transformed cells various electrophoresis techniques PCR protocol etcThe appendices in the last part are included to provide information important to he study of the above-mentioned practical as a wholeThe book will be useful to students belonging to Biotechnology agriculture and allied fields The idea behind this practical manual was thus to provide theoretical basis of the practical study items to be undertaken in the laboratory in a lucid manner 254 pp. Bestandsnummer des Verkäufers 88665
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