Verwandte Artikel zu Structure and Physics of Viruses: An Integrated Textbook
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Inhaltsangabe
Foreword
Preface
Contributors
Part I. The viral machine
1. Introduction: the structural basis of virus function
1.1 The structure and physics of viruses
1.2 Virions and their structural components
1.3 Techniques used to study the structure and physics of viruses
1.4 The roles of virus particles and their components along the virus life cycle
1.5 Experimental and theoretical developments in physical virology
1.6 Applied structural and physical virology
1.7. Concluding remarks
Acknowledgements
References and further reading
2. The basic architecture of viruses
2.1 Introduction
2.2 How virus structures are studied
2.3 Viral capsid symmetry
2.4 Quasi-equivalence theory and icosahedral capsid architecture
2.5 Variations on the icosahedral capsid theme: multiple layers and prolate icosahedra
2.6 Helical capsids
2.7 The viral nucleic acid inside
2.8 Basic architecture of enveloped viruses
Acknowledgements
References and further reading
Part II. Determination of the structure and physical properties of viruses
3. Conventional electron microscopy, cryo-electron microscopy and cryo-electron tomography of viruses
3.1 Introduction
3.2 Transmission electron microscopy of viruses
3.3 Cryo-electron microscopy of viruses
3.4 Cryo-EM image processing and three-dimensional reconstruction
3.5 Near-atomic resolution of virus structures by cryo-EM3.6 Reconstructing viruses without imposing symmetry
3.7 Reconstructing viruses with helical symmetry
3.8 Cryo-electron tomography of viruses
3.9 Understanding viruses: some major contributions of electron microscopy and tomography
3.10 Perspectives
Acknowledgements
References and further reading
4. X-ray crystallography of viruses
4.1 Introduction
4.2 Basic concepts and general experimental design
4.3 Production and purification of viral particles and proteins for structural studies
4.4 Crystallization
4.5 Data collection and processing
4.6 Phase determination
4.7 Map interpretation
4.8 Understanding viruses: some major contributions of X-ray crystallography
4.9 Perspectives
Acknowledgements
References and further reading
5. Nuclear magnetic resonance spectroscopy to study virus structure
5.1 Introduction
5.2 Physical principles of NMR spectroscopy
5.3 Determination of biomolecular structures by NMR spectroscopy
5.4 NMR structures of viral macromolecules
5.5 Understanding viruses: some major contributions of NMR spectroscopy
5.6 Perspectives
Acknowledgements
References and further reading
6. Fluorescence, circular dichroism and mass spectrometry as tools to study virus structure
6.1 Introduction
6.2 Physical principles of fluorescence and circular dichroism (CD) spectroscopies
6.3 Fluorescence and CD spectroscopies to study virus structure
6.4 Mass spectrometry (MS) as an analytical tool
6.5 MS to study virus structure
6.6 Perspectives
Acknowledgements
References and further reading
7. Combined approaches to study virus structure
7.1 Introduction: the 'multi-disciplinary approach' concept in structural virology
7.2 Some classical methods in structural virology: a brief overview
7.3 Combining X-ray crystallography and electron microscopy
7.4 Dissecting virus structures by combining biochemical, genetic and biophysical tools
7.5 Combining electron microscopy and electron tomography
7.6 From virus in solution to virus in cells
7.7 Emerging hybrid methods
7.8 The biology behind the combined methods
Acknowledgements
References and further reading
8. Atomic force microscopy of viruses
8.1 Introduction
8.2 Basic concepts
8.3 AFM implementation
8.4 Imaging viruses and other biological objects
8.5 Understanding viruses: some major contributions of AFM
8.6 Perspectives
Acknowledgements
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