Reverse Phase Protein Arrays: From Technical and Analytical Fundamentals to Applications (Advances in Experimental Medicine and Biology, 1188, Band 1188) - Hardcover

Hardcover

ISBN 10: 9813297549 ISBN 13: 9789813297548

Verlag: Springer, 2019

Inhaltsangabe

This book provides practical guidance on all aspects of reverse phase protein array (RPPA) technology, which permits the quantification of protein levels in cell or tissue lysates. In addition, the latest results are presented from laboratories across the world where experts are successfully running the challenging RPPA data platform. After an introductory chapter by the inventor of the RPPA technique, the demanding task of lysate preparation is explained. Subsequent chapters review printing platforms and analytical platforms and discuss antibody screening in detail. The role of RPPA data in integrative analyses is thoroughly examined, and the various applications of RPPA, for example to identify molecular targeting drugs or evaluate cancer drug efficacy, are discussed with reference to the latest knowledge. It is hoped that the book will foster the implementation of RPPA by documenting the practical details of the technology and by revealing its great potential. It will be of high value for researchers who plan to build an RPPA platform in their institute and for researchers, clinicians, and companies who use RPPA through collaboration with an existing RPPA facility.

Über diesen Titel

Über die Autorin bzw. den Autor

Tesshi Yamada, M.D., Ph.D. 

Professor of International University of Health and Welfare, Narita, Japan

Satoshi Nishizuka, M.D., Ph.D. 

Professor of Iwate Medical University, Japan

Gordon Mills, M.D., Ph.D. 

Professor of University of Texas, MD Anderson Cancer Center

Lance Liotta, M.D., Ph.D. 

Co-Director of the Center for Applied Proteomics and Molecular Medicine, George Mason University

Von der hinteren Coverseite

This book provides practical guidance on all aspects of reverse phase protein array (RPPA) technology, which permits the quantification of protein levels in cell or tissue lysates. In addition, the latest results are presented from laboratories across the world where experts are successfully running the challenging RPPA data platform. After an introductory chapter by the inventor of the RPPA technique, the demanding task of lysate preparation is explained. Subsequent chapters review printing platforms and analytical platforms and discuss antibody screening in detail. The role of RPPA data in integrative analyses is thoroughly examined, and the various applications of RPPA, for example to identify molecular targeting drugs or evaluate cancer drug efficacy, are discussed with reference to the latest knowledge. It is hoped that the book will foster the implementation of RPPA by documenting the practical details of the technology and by revealing its great potential. It will be of high value for researchers who plan to build an RPPA platform in their institute and for researchers, clinicians, and companies who use RPPA through collaboration with an existing RPPA facility.

Details zur Ausgabe

• Verlag: Springer
• Erscheinungsdatum: 2019
• Sprache: Englisch
• ISBN 10: 9813297549
• ISBN 13: 9789813297548
• Einband: Gebundene Ausgabe
• Auflage: 1
• Anzahl der Seiten: 274
• Herausgeber: Yamada Tesshi, Nishizuka Satoshi S., Mills Gordon B., Liotta Lance A.
• Kontakt zum Hersteller: Nicht verfügbar
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