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Applied Biophotonics and Optical Biosensors: A Practical Guide to Biomedical Imaging, Fluorescence Microscopy, Spectroscopy, Laser Diagnostics, and Optical Sensor Design - Softcover
Inhaltsangabe
Light can measure blood oxygen through a fingertip, image the layers of the retina in milliseconds, and register protein binding on a thin film of gold. Turning that physics into an instrument that works, and that people can trust, is a different challenge.
Most engineers meet biophotonics in pieces: optics in one course, tissue biology in another, and detectors, signal processing, and surface chemistry somewhere else. That leaves a hard gap between knowing the principles and being able to choose a wavelength, size a fiber probe, estimate a detection limit, or show that a measurement deserves trust.
Applied Biophotonics and Optical Biosensors closes that gap with one connected, quantitative path from the behavior of light in living tissue to the design, analysis, and validation of working instruments and sensors. Every chapter develops the theory from stated assumptions and applies it in worked examples that end in a checked answer with units.
With this book, you will learn how to:
- Predict how deeply light penetrates tissue and how much returns, using diffusion theory and photon-transport simulation.
- Select light sources, photodetectors, lenses, fibers, filters, and spectrometers to reach a required signal-to-noise ratio.
- Compare spectroscopy, microscopy, coherence imaging, and deep-tissue methods by depth, resolution, and specificity before committing to a design.
- Design pulse oximeters and wearable optical sensors, including calibration, accuracy, and the effect of skin pigmentation.
- Develop label-free biosensors on plasmonic, waveguide, resonator, and fiber platforms and estimate their detection limits.
- Plan analytical validation, optical radiation safety, and the United States regulatory pathway from the earliest design stage.
Inside the book:
- Foundations: optical principles, tissue optics, light-transport models, sources and detectors, and optical instrument design.
- Methods: absorption, fluorescence, and vibrational spectroscopy; widefield to super-resolution microscopy; optical coherence tomography; near-infrared and photoacoustic sensing; and physiological monitoring.
- Biosensors and translation: biorecognition and binding kinetics; plasmonic, integrated photonic, and fiber-optic sensors; and point-of-care integration, validation, safety, and regulation.
- 16 chapters, 214 worked examples, 160 practice problems with answers, 50 original figures, and a 280-term glossary.
Written for upper-level undergraduate and graduate students in biomedical engineering, physics, and related fields, and for engineers and scientists who design, specify, or evaluate optical systems for medical devices, wearables, imaging, and diagnostics. Calculus and introductory optics are assumed; everything else is developed in the text. Suggested reading paths support one-semester courses in biomedical optics or optical biosensors.
Get your copy and start designing optical instruments and biosensors with a clear, quantitative understanding of what light can and cannot measure.
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Applied Biophotonics and Optical Biosensors: A Practical Guide to Biomedical Imaging, Fluorescence Microscopy, Spectroscopy, Laser Diagnostics, and Optical Sensor Design
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