Application of Selected Reaction Monitoring to Highly Multiplexed Targeted Quantitative Proteomics (Paperback)

Sprache: Englisch

Verlag: Springer-Verlag New York Inc., New York, NY, 2013

1461486653 / 9781461486657

Serie: Buch 5 von 12 - SpringerBriefs in Systems Biology

Anbieter: AussieBookSeller, Truganina, VIC, AustralienAussieBookSeller

Verkäufer/-in mit 5 Sternen

AbeBooks-Verkäufer/-in seit 22. Juni 2007

Artikel dieses Verkäufers ansehen
Softcover

Zustand: Neu

EUR 103,57

EUR 31,86 Versand 
Versand von Australien nach USA

Anzahl: 1 verfügbar

In den Warenkorb
Kostenlose Rückgaben innerhalb von 30 Tagen

Artikelbeschreibung vom Verkäufer

Paperback. A key experiment in biomedical research is monitoring the expression of different proteins in order to detect changes that occur in biological systems under different experimental conditions. The method that is most widely used is the Western blot analysis. While Western blot is a workhorse in laboratories studying protein expression and has several advantages, it also has a number of significant limitations. In particular, the method is semi-quantitative with limited dynamic range. Western blot focuses on a single protein per sample with only a small number of representative samples analyzed in an experiment. New quantitative tools have been needed for some time to at least supplement, & possibly replace, the Western blot. Mass spectrometric methods have begun to compete with Western blot for routine quantitative analyses of proteins. One of these methods is based on the tandem mass spectrometry technique of selected reaction monitoring (SRM), which is also called multiple reaction monitoring (MRM). Selected reaction monitoring is actually an older tandem mass spectrometry technique, first described in the late 70s, that is widely utilized in the quantitative analysis of small molecules like drugs & metabolites. The use of selected reaction monitoring for the quantitative analysis of proteins has a number of advantages. Most importantly, it is fundamentally quantitative with a wide dynamic range. The output of the analysis is a numerical result that can range over several orders of magnitude. Other advantages include sufficient specificity & sensitivity to detect low abundance proteins in complex mixtures. Finally, selected reaction monitoring can be multiplexed to allow the quantitative analysis of relatively large numbers of proteins in a single sample in a single experiment. This Brief will explain both the theoretical & experimental details of the selected reaction monitoring experiment as it is appliedto proteins. A key experiment in biomedical research is monitoring the expression of different proteins in order to detect changes that occur in biological systems under different experimental conditions. Shipping may be from our Sydney, NSW warehouse or from our UK or US warehouse, depending on stock availability.

Bestandsnummer des Verkäufers 9781461486657

Titel
Application of Selected Reaction Monitoring to Highly Multiplexed Targeted Quantitative Proteomics (Paperback)
Autor
Michael Kinter
Verlag
Springer-Verlag New York Inc., New York, NY
Veröffentlichungsjahr
2013
Zustand
new
Einband
Paperback
Sprache
Englisch
ISBN-10
1461486653
ISBN-13
9781461486657
Serie
Buch 5 von 12: SpringerBriefs in Systems Biology

AussieBookSeller

Truganina, VIC, Australien

Verkäufer/-in mit 5 Sternen

AbeBooks-Verkäufer/-in seit 22. Juni 2007

Versandkosten von Australien nach USA

Artikel25 bis 45 Werktage8 bis 14 Werktage
Erster ArtikelEUR 31,86EUR 37,89
Die Versandzeiten werden von den Verkäuferinnen und Verkäufern festgelegt. Sie variieren je nach Versanddienstleister und Standort. Sendungen, die den Zoll passieren, können Verzögerungen unterliegen. Eventuell anfallende Abgaben oder Gebühren sind von der Käuferin bzw. dem Käufer zu tragen. Die Verkäuferin bzw. der Verkäufer kann Sie bezüglich zusätzlicher Versandkosten kontaktieren, um einen möglichen Anstieg der Versandkosten für Ihre Artikel auszugleichen.

Zahlungsarten

  • Visa
  • Mastercard
  • American Express
  • Carte Bleue
  • Apple Pay
  • Google Pay

Unternehmensdaten der Verkäuferin bzw. des Verkäufers

The Nile Group Pty Ltd

42 Apex Drive
Truganina, VIC Australien 3029