A Beginner's Guide to Microarrays

Eric M. Blalock

ISBN 10: 1461346843 / ISBN 13: 9781461346845

Verlag: Springer US, Springer New York Sep 2012, 2012

Beschreibung

This item is printed on demand - Print on Demand Titel. Neuware -A Beginner's Guide to Microarrays addresses two audiences - the core facility manager who produces, hybridizes, and scans arrays, and the basic research scientist who will be performing the analysis and interpreting the results. User friendly coverage and detailed protocols are provided for the technical steps and procedures involved in many facets of microarray technology, including:Cleaning and coating glass slidesDesigning oligonucleotide probesConstructing arrays for the detection and quantification of different bacterial speciesPreparing spotting solutionsTroubleshooting spotting problemsSetting up and running a core facilityNormalizing background signal and controlling for systematic varianceDesigning experiments for maximum effectAnalyzing data with statistical proceduresClustering data with machine-learning protocols.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 368 pp. Englisch.

Bestandsnummer des Verkäufers 9781461346845

Bibliografische Details

• Titel: A Beginner's Guide to Microarrays
• Verlag: Springer US, Springer New York Sep 2012
• Erscheinungsjahr: 2012
• Sprache: Englisch
• ISBN-10: 1461346843
• ISBN-13: 9781461346845
• Einband: Taschenbuch
• Zustand: Neu

Über diesen Titel

Inhaltsangabe

A Beginner's Guide to Microarrays addresses two audiences - the core facility manager who produces, hybridizes, and scans arrays, and the basic research scientist who will be performing the analysis and interpreting the results. User friendly coverage and detailed protocols are provided for the technical steps and procedures involved in many facets of microarray technology, including:

-Cleaning and coating glass slides,
-Designing oligonucleotide probes,
-Constructing arrays for the detection and quantification of different bacterial species,
-Preparing spotting solutions,
-Troubleshooting spotting problems,
-Setting up and running a core facility,
-Normalizing background signal and controlling for systematic variance,
-Designing experiments for maximum effect,
-Analyzing data with statistical procedures,
-Clustering data with machine-learning protocols. 

Críticas

From the reviews:

"The book offers a broad coverage of many of the most important aspects of microarray technology ... . Despite the wide field broached by this book, one of its chief successes lies with the fact that in each chapter the authors provide a clear review of every step of the technique, including both its potential and pitfalls. This allows all readers, especially beginners, to understand the development and impact of this important technique. ... Readers will find this beginner’s guide book very informative ... ." (Guillermina Lopez-Bendito, Journal of Chemical Neuroanatomy, Vol. 30, 2005)