CRISPR-Cas Methods

M. Tofazzal Islam

ISBN 10: 1071606158 / ISBN 13: 9781071606155

Verlag: Springer US, Springer US, 2020

Beschreibung

Druck auf Anfrage Neuware - Printed after ordering - This volume details the fundamentals of the CRISPR-Cas system, and its protocols illustrate advances in CRISPR-Cas techniques for efficient genome editing. Introductory chapters provide a wide horizon of CRISPR/Cas-based methods and applications. Additional chapters guide readers through HDR-mediated editing, sgRNA design, the step-by-step procedure of multiplex adenine base editing experiments in rice, generating mutants for rice, wheat, Brachypodium, Barley, Flax, and Phytophthora, visual screening of mutants, gene deletion (knock-out), tagging (knock-in) in mammalian cells, the cloning-free (DNA-free) technique, cell-penetrating peptides, generating a genome-edited banana, and nuclear genome editing of Chlamydomonas employing CRISPR-Cpf1 combined with a single-stranded DNA (ssODN) repair template.Authoritative and cutting-edge, CRISPR-Cas Methods aims to assist researchers who are new to the field and are aiming to learn how best to adopt this technology for a particular organism.

Bestandsnummer des Verkäufers 9781071606155

Bibliografische Details

• Titel: CRISPR-Cas Methods
• Verlag: Springer US, Springer US
• Erscheinungsjahr: 2020
• Sprache: Englisch
• ISBN-10: 1071606158
• ISBN-13: 9781071606155
• Einband: Buch
• Zustand: Neu
• Abmessungen: 260B x 183 H x 22 T Millimeter Breite: 260 mm, Höhe: 183 mm, Tiefe: 22 mm
• Gewicht: 758 Gramm

Über diesen Titel

Inhaltsangabe

This volume details the fundamentals of the CRISPR-Cas system, and its protocols illustrate advances in CRISPR-Cas techniques for efficient genome editing. Introductory chapters provide a wide horizon of CRISPR/Cas-based methods and applications. Additional chapters guide readers through HDR-mediated editing, sgRNA design, the step-by-step procedure of multiplex adenine base editing experiments in rice, generating mutants for rice, wheat, Brachypodium, Barley, Flax, and Phytophthora, visual screening of mutants, gene deletion (knock-out), tagging (knock-in) in mammalian cells, the cloning-free (DNA-free) technique, cell-penetrating peptides, generating a genome-edited banana, and nuclear genome editing of Chlamydomonas employing CRISPR-Cpf1 combined with a single-stranded DNA (ssODN) repair template. 
Authoritative and cutting-edge, CRISPR-Cas Methods aims to assist researchers who are new to the field and are aiming to learn how best to adopt this technology for a particular organism.

Über die Autorin bzw. den Autor