CRISPR Gene Editing: Methods and Protocols (Methods in Molecular Biology, 1961)

ISBN 10: 1493991698 / ISBN 13: 9781493991693

Verlag: Humana, 2019

Beschreibung

Bestandsnummer des Verkäufers ABLIING23Mar2716030188417

Bibliografische Details

Titel: CRISPR Gene Editing: Methods and Protocols (...

Verlag: Humana

Erscheinungsdatum: 2019

Einband: Hardcover

Zustand: New

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Inhaltsangabe:

This detailed volume guides readers through strategic planning and user-friendly guidelines in order to select the most suitable CRISPR-Cas system and target sites with high activity and specificity. Methods covering CRISPR gRNA design, CRISPR delivery, CRISPR activity quantification (indel quantification), and examples of applying CRISPR gene editing in human pluripotent stem cells, primary cells, gene therapy, and genetic screening are included. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and invaluable, CRISPR Gene Editing: Methods and Protocols will assist undergraduates, graduates, and researchers with detailed guidelines and methods for the vitally important CRISPR gene editing field. 

Chapter 3 is available open access under a CC BY 4.0 license via link.springer.com.