Part I: General
1. Heterogeneity of Metazoan Cells and Beyond: To Integrative Analysis of Cellular Populations at Single-Cell Level
Natasha S. Barteneva and Ivan A. Vorobjev
2. Integrating Analysis of Cellular Heterogeneity in High Content Dose-Response Studies
Albert Gough, Tong Ying Shun, D. Lansing Taylor, and Mark Schurdak
3. Image-Based Tracking of Heterogeneous Single Cell Phenotypes
Katherin Patsch, Shannon M. Mumenthaler, and Daniel Ruderman
Part II: Cytometry in Cellular Heterogeneity Research
4. High-Dimensional Single Cell Analysis with Mass Cytometry
Tess Melinda Brodie and Vinko Tosevski
5. Spectral and Imaging Flow Cytometry in Phytoplankton Research
Veronika Dashkova, Jeff Clapper, Ivan A. Vorobjev, and Natasha S. Barteneva
6. X-Ray Fluorescence Detected Flow Cytometry
Andrew M. Crawford and James E. Penner-Hahn
7. Multiparametric Analysis of Myeloid Populations by Flow Cytometry
Sara A. Mathie, Alastair L. Corbin, Hayley L. Eames, and Irina A. Udalova
8. Quantitation of IRF3 Nuclear Translocation in Heterogeneous Cellular Populations from Cervical Tissue Using Imaging Flow Cytometry
Radiana T. Trifonova and Natasha S. Barteneva
9. Methods of Study of Neuron Structural Heterogeneity: Flow Cytometry vs. Laser Interferometry
Ekaterina Kopeikina, Marina Dukhinova, and Eugene D. Ponomarev
10. Usage of Multiparameter Flow Cytometry to Study Microglia and Macrophage Heterogeneity in the Central Nervous System during Neuroinflammation and Neurodegeneration
Marina Dukhinova, Ekaterina Kopeikina, and Eugene D. Ponomarev
Part III: Imaging and Spectroscopy Methods in Heterogeneity Studies
11. Analysis of Microtubule Dynamics Heterogeneity in Cell Culture
Anara Serikbaeva, Anna Tvorogova, Sholpan Kauanova, and Ivan A. Vorobjev
12. Heterogeneity of Focal Adhesions and Focal Contacts in Motile Fibroblasts
Aleena Gladkikh, Anastasia Kovaleva, Anna Tvorogova, and Ivan A. Vorobjev
13. Laser Tweezers Raman Micro-Spectroscopy of Single Cells and Biological Particles
Maria Navas-Moreno and James W. Chan
Part VI: Metabolic and Molecular Biological Methods in Cell Heterogeneity Tracking
14. Quantification of the Metabolic Heterogeneity in Mycobacterial Cells Through the Measurement of the NADH/NAD+ Ratio Using a Genetically-Encoded Sensor
Shabir Ahmad Bhat, Iram Khan Iqbal, and Ashwani Kumar
15. Characterizing Cell Heterogeneity Using PCR Fingerprinting of Surface Multigene Families in Protozoan Parasites
Víctor Seco-Hidalgo, Antonio Osuna, and Luis Miguel de Pablos
16. Assessing Carbon Source-Dependent Phenotypic Variability in Pseudomonas putida
Pablo I. Nikel and Víctor de Lorenzo
Part V: Chromatin and Cell Cycle Heterogeneity
17. The Retinal Pigment Epithelial Cell Line (ARPE-19) Displays Mosaic Structural Chromosomal Aberrations
Elizaveta Fasler-Kan, Nijas Aliu, Kerstin Wunderlich, Sylvia Ketterer, Sabrina Ruggiero, Steffen Berger, and Peter Meyer
18. FACS Isolation of Viable Cells in Different Cell Cycle Stages from Asynchronous Culture for RNA-Sequencing
Daria M. Potashnikova, Sergey A. Golyshev, Alexey A. Penin, Maria D. Logacheva, Anna V. Klepikova, Anastasia A. Zharikova, Alexey A. Mironov, Eugene V. Sheval, and Ivan A. Vorobjev
19. Measuring Nanoscale Chromatin Heterogeneity with Parti
This volume is essential for anyone struggling with the cellular heterogeneity field, and it connects this new area with the latest advances in innovative techniques combined with practical advice from international experts. The chapters provide a comprehensive overview of cellular heterogeneity analysis techniques, exploring cytometry, imaging and spectroscopy methods, metabolic and molecular biological methods in tracking, as well as chromatin and cell cycle heterogeneity. Written in the highly successful
Methods in Molecular Biology series format, chapters include introductions to their respective topics, detailed lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and accessible, Cellular Heterogeneity: Methods and Protocols encourages readers to explore new ways of studying cells that will help lead to exciting new discoveries.
