Chemical, Kinetic, and Spectral Properties of the Catalytic Mechanism of Galactose Oxidase: A Dissertation Submitted to the State University of New York At Buffalo in Partial Fulfillment for the Degree of Doctor of Philosophy

Paul S. Tressel

Verlag: State University of New York at Buffalo, 1980

Beschreibung

1980 first edition, Faculty of of the Graduate School of the State University Of New York (Buffalo), 8 x 11 inches tall, burgundy hardcover with silver lettering to front cover and spine, 164 pp. Doctoral dissertation from S.U.N.Y. Buffalo. Typewritten manuscript. Front cover is mildly rubbed and has one tiny white spot. Corners are bumped. Otherwise, an excellent copy of this resource - clean, bright and unmarked. Filled with equations and tables with list of figures and abbreviations. ~OO~ Horseradish peroxidase (HRP) catalytically modifies galactose oxidase (GOase) to cause an increase in GOase activity, visible absorption intensity and CD spectral intensity, but no change in the GOase EPR signal. The modification is oxygen dependent, labile and involves the formation of a GOase free radical (as evidenced by an ability to trap a species of modified GOase that gives a unique amino acid analysis peak, corresponding to o-o dityrosine). The nature of modification is complicated; it seems to involve a free radical oxygen species, its extent varies inversely with GOase concentration, it affects the GOase activity and spectral intensity at different rates, and can reverse the inhibitory effects of tyrosine modification by tyrosinase. (From the Abstract) Contents: Introduction; Distances from Copper to Functional Groups; Kinetic and Spectral Studies of Exogenous Copper Ligands; The Interaction Between HRP and GOase; Substrate Studies; Buffer Anion Studies; K3Fe(CN)6; Appendices; Summary of Accomplishments; Bibliography. Bestandsnummer des Verkäufers 4Q-PF6O-MCHB

Bibliografische Details

Titel: Chemical, Kinetic, and Spectral Properties ...

Verlag: State University of New York at Buffalo

Erscheinungsdatum: 1980

Einband: Hardcover

Zustand: Very Good