ELIS- ENZYME LINKED IMMUNOSORBENT ASSA
IQBAL,AMJAD
ISBN 10: 3848436639 ISBN 13: 9783848436637
Verlag: LAP LAMBERT Academic Publishing, 2012
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Softcover
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Beschreibung
Beschreibung:
Bestandsnummer des Verkäufers NWUS9783848436637
Inhaltsangabe:
In ELISA formats there are several parameters that are common and critical to assay performance and should be considered during the optimization process, including antibodies concentration, antigen concentration, incubation time of blocking, substrate solution and washing solution. Though immunoassays offer a specific, sensitive and rapid method to detect and quantify even trace amounts of allergens in foodstuffs, certain processes can denature, alter or destroy proteins so that they are no longer detectible by assay using antibodies. When using monoclonal antibodies, there is a great chance of obtaining false negatives in the detection. Polyclonal antibodies are recommended for ELISA techniques as the risk of obtaining false negatives is reduced as compared to monoclonal antibodies which are quite specific.
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Bibliografische Details
Titel: ELIS- ENZYME LINKED IMMUNOSORBENT ASSA
Verlag: LAP LAMBERT Academic Publishing
Erscheinungsdatum: 2012
Einband: Softcover
Zustand: NEW