Electron Crystallography of Soluble and Membrane Proteins: Methods and Protocols: 955 (Methods in Molecular Biology, 955)

Schmidt-Krey, Ingeborg

ISBN 10: 1627031758 / ISBN 13: 9781627031752

Verlag: Humana, 2012

This Methods in Molecular Biology(TM) book covers techniques used in electron crystallography, including protein sample preparation, 2D crystallization and more. Offers the detailed descriptions and implementation advice needed to ensure successful results. Editor(s): Schmidt-Krey, Ingeborg; Cheng, Yifan. Series: Methods in Molecular Biology. Num Pages: 586 pages, 12 black & white tables, biography. BIC Classification: PSBC. Category: (P) Professional & Vocational. Dimension: 192 x 292 x 39. Weight in Grams: 1308. . 2012. 2013th Edition. hardcover. . . . . Books ship from the US and Ireland. Bestandsnummer des Verkäufers V9781627031752

Inhaltsangabe:

The basic principle of electron crystallography is to calculate a 3D density map by combining the amplitudes obtained from electron diffraction patterns with the experimental phases calculated from images of two-dimensional crystals of membrane or soluble proteins. This technology is very well developed and has produced a number of atomic models of membrane proteins in a lipid environment.  Focused on comprehensive experimental protocols, Electron Crystallography of Soluble and Membrane Proteins: Methods and Protocols covers the entire range of techniques used in electron crystallography, including protein sample preparation, 2D crystallization, and screening in negative stain over electron cryo-microscopy (cryo-EM) and data processing, as well as modeling of conformational changes. Additional chapters provide perspective on past, present, and future challenges as well as complementary methods.  Written for the popular Methods in Molecular Biology™ series, the work contains the kind of detailed descriptions and implementation advice necessary to ensure successful results.

 

Comprehensive and cutting-edge, Electron Crystallography of Soluble and Membrane Proteins: Methods and Protocols serves laboratories new to the methods as well as state-of-the-art facilities pursuing this exciting area of protein science.

Von der hinteren Coverseite:

The basic principle of electron crystallography is to calculate a 3D density map by combining the amplitudes obtained from electron diffraction patterns with the experimental phases calculated from images of two-dimensional crystals of membrane or soluble proteins. This technology is very well developed and has produced a number of atomic models of membrane proteins in a lipid environment. Focused on comprehensive experimental protocols, Electron Crystallography of Soluble and Membrane Proteins: Methods and Protocols covers the entire range of techniques used in electron crystallography, including protein sample preparation, 2D crystallization, and screening in negative stain over electron cryo-microscopy (cryo-EM) and data processing, as well as modeling of conformational changes. Additional chapters provide perspective on past, present, and future challenges as well as complementary methods. Written for the popular Methods in Molecular Biology™ series, the work contains the kind of detailed descriptions and implementation advice necessary to ensure successful results.

Comprehensive and cutting-edge, Electron Crystallography of Soluble and Membrane Proteins: Methods and Protocols serves laboratories new to the methods as well as state-of-the-art facilities pursuing this exciting area of protein science.

Bibliografische Details

Titel: Electron Crystallography of Soluble and ...
Verlag: Humana
Erscheinungsdatum: 2012
Einband: Hardcover
Zustand: New