Fluorescent Protein-Based Biosensors : Methods and Protocols

Jin Zhang

ISBN 10: 1627036210 / ISBN 13: 9781627036214

Verlag: Humana Press, Humana Press, 2013

Beschreibung

nach der Bestellung gedruckt Neuware - Printed after ordering - In Fluorescent Protein-Based Biosensors: Methods and Protocols, experts in the field have assembled a series of protocols describing several methods in which fluorescent protein-based reporters can be used to gain unique insights into the regulation of cellular signal transduction. Genetically encodable fluorescent biosensors have allowed researchers to observe biochemical processes within the endogenous cellular environment with unprecedented spatiotemporal resolution. As the number and diversity of available biosensors grows, it is increasingly important to equip researchers with an understanding of the key concepts underlying the design and application of genetically encodable fluorescent biosensors to live cell imaging. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Fluorescent Protein-Based Biosensors: Methods and Protocols promises to be a valuable resource for researchers interested in applying current biosensors to the study of biochemical processes in living cells as well as those interested in developing novel biosensors to visualize other cellular phenomena.

Bestandsnummer des Verkäufers 9781627036214

Bibliografische Details

• Titel: Fluorescent Protein-Based Biosensors : Methods and Protocols
• Verlag: Humana Press, Humana Press
• Erscheinungsjahr: 2013
• Sprache: Englisch
• ISBN-10: 1627036210
• ISBN-13: 9781627036214
• Einband: Buch
• Zustand: Neu
• Abmessungen: 260B x 183 H x 19 T Millimeter Breite: 260 mm, Höhe: 183 mm, Tiefe: 19 mm
• Gewicht: 700 Gramm

Über diesen Titel

Inhaltsangabe

In Fluorescent Protein-Based Biosensors: Methods and Protocols, experts in the field have assembled a series of protocols describing several methods in which fluorescent protein-based reporters can be used to gain unique insights into the regulation of cellular signal transduction. Genetically encodable fluorescent biosensors have allowed researchers to observe biochemical processes within the endogenous cellular environment with unprecedented spatiotemporal resolution. As the number and diversity of available biosensors grows, it is increasingly important to equip researchers with an understanding of the key concepts underlying the design and application of genetically encodable fluorescent biosensors to live cell imaging. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Fluorescent Protein-Based Biosensors: Methods and Protocols promises to be a valuable resource for researchers interested in applying current biosensors to the study of biochemical processes in living cells as well as those interested in developing novel biosensors to visualize other cellular phenomena.