Genetically Incorporated Non-Canonical Amino Acids: Methods and Protocols

ISBN 10: 1071632531 / ISBN 13: 9781071632536

Verlag: Humana, 2023

Beschreibung

Print on Demand.

Bestandsnummer des Verkäufers 396281754

Bibliografische Details

• Titel: Genetically Incorporated Non-Canonical Amino Acids: Methods and Protocols
• Verlag: Humana
• Erscheinungsjahr: 2023
• Sprache: Englisch
• ISBN-10: 1071632531
• ISBN-13: 9781071632536
• Einband: Softcover
• Zustand: New

Über diesen Titel

Inhaltsangabe

This detailed volume explores non-canonical amino acids (ncAAs) through their site-specific incorporation by genetic code expansion (GCE). The collection provides a broad resource of methods for implementing GCE in E. coli, mammalian cells, and animals, highlighting specific applications ranging from fluorescence labeling to photocontrol and the study of protein post-translational modification. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Genetically Incorporated Non-Canonical Amino Acids: Methods and Protocols serves as an ideal source of methodologies that can be adapted and extended, migrated to different model systems, and combined in new ways to help explore a wide range of biological questions and to augment industrial and pharmaceutical protein engineering.