Documenting the exponential growth in cryo-electron microscopy, the resulting advances, new hypotheses, and heightened understanding of the ultrastructure of living and inert matter, this book employes an accessible, step-by-step format to present the most sophisticated methods used in research laboratories today. It describes the basic principles of cryo-fixation, cryo-ultramicrotomy, embedding at low temperatures, and freeze-fracture. It explores applications in electron crystallography, 2D/3D structure analysis, cryo-electron tomography, and techniques for protein localization. Each chapter covers a specific method, including detailed protocols, summary tables, and hundreds of illustrations and diagrams.

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Annie Cavalier

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