Molecular and Biochemical Analysis of Calreticulin | Signal Transduction and MAPK pathways

Fatme Lezzeik

ISBN 10: 3659337269 / ISBN 13: 9783659337260

Verlag: LAP LAMBERT Academic Publishing, 2015

Molecular and Biochemical Analysis of Calreticulin | Signal Transduction and MAPK pathways | Fatme Lezzeik | Taschenbuch | 112 S. | Englisch | 2015 | LAP LAMBERT Academic Publishing | EAN 9783659337260 | Verantwortliche Person für die EU: preigu GmbH & Co. KG, Lengericher Landstr. 19, 49078 Osnabrück, mail[at]preigu[dot]de | Anbieter: preigu. Bestandsnummer des Verkäufers 104791078

Inhaltsangabe:

Calreticulin (CRT) deals with misfolded proteins by binding to them and marking them for degradation so that only the properly folded proteins are allowed to move away from the ER. It is predicted that a downstream mitogen-activated protein kinase may mediate this phosphorylation. Therefore, salicylic acid treatment was used to enhance the expression in CRT gene in tomato. The activity of ERK sub-group of MAPK can be inhibited by ERK inhibitors. The ERK inhibitor interrupted the physical interaction of ERK-type MAPKs and their upstream MAPKKs. Unfolded protein response (UPR) caused by the ER stress was induced by tunicamycin (TM) or dithiothreitol (DTT). Expression cloning of CRT in E.coli was conducted and the expression of CRT protein was examined by SDS-PAGE and Western blotting. Two enzymes, ß-1, 3-glucanase (PR2) and glutamine synthetase, were also examined under stress treatment. It is hoped that this work would produce new knowledge into diverse aspects of MAPK pathways to facilitate comprehensive and molecular understanding of the complex defense system. The discoveries will lay the ground for knowledge-based innovative strategies to reduce the impact of plant diseases.

Über die Autorin bzw. den Autor: Fatme Lezzeik, MSc: Studied Biology at Carleton University, Canada. Biology laboratory instructor at Lebanese International University, Lebanon.

Bibliografische Details

Titel: Molecular and Biochemical Analysis of ...
Verlag: LAP LAMBERT Academic Publishing
Erscheinungsdatum: 2015
Einband: Taschenbuch
Zustand: Neu