Multimerization strategy to create an artificial pore-forming protein

Claudia A. Fichera (u. a.)

ISBN 10: 3639663004 / ISBN 13: 9783639663006

Verlag: Scholars' Press, 2014

Multimerization strategy to create an artificial pore-forming protein | Claudia A. Fichera (u. a.) | Taschenbuch | 64 S. | Englisch | 2014 | Scholars' Press | EAN 9783639663006 | Verantwortliche Person für die EU: preigu GmbH & Co. KG, Lengericher Landstr. 19, 49078 Osnabrück, mail[at]preigu[dot]de | Anbieter: preigu. Bestandsnummer des Verkäufers 105080184

Inhaltsangabe:

Porines generate little or no selective water channels, sometimes voltage-dependent, and several of them play important roles in specific cellular pathways. Through a preliminary bioinformatic analysis was identified a pattern of basic protein with a hairpin structure (β-strand/loop/β-strand) from the PA83 protein of Bacillus anthracis. Through the realization of a very quick and simple experimental protocol we built an artificial construct with seven multimers of the basic module. The experimental plan developed allowed us to obtain in a few days a chimeric protein completely artificial, which was cloned into a bacterial expression vector. Expression experiments has carried out interesting results since we observed that the induced bacterial cultures were less cloudy, indicating a reduced cell growth. In accordance with the recent literature we are inclined to believe that the protein acts like antibiotic, fitting massively in the membrane and causing cell lysis of the host bacteria. This strategy could be used for the production of artificial proteins with biological, pharmaceutical and industrial high-impact.

Über die Autorin bzw. den Autor: Dr. Fichera Claudia A. graduated in Biological Sciences in 2001. In 2006 she specialized in Microbiology and then she worked as researcher in several italian and foreign laboratories (London and Germany). In 2014 she obtained her PhD in Biotechnology at University of Catania. She currently teaches Math and Science to public school.

Bibliografische Details

Titel: Multimerization strategy to create an ...
Verlag: Scholars' Press
Erscheinungsdatum: 2014
Einband: Taschenbuch
Zustand: Neu