Pichia Protocols (Methods in Molecular Biology)

David R. Higgins; James M. Cregg

ISBN 10: 0896034216 / ISBN 13: 9780896034211

Verlag: Humana Press, 1998

May have limited writing in cover pages. Pages are unmarked. ~ ThriftBooks: Read More, Spend Less 1.45. Bestandsnummer des Verkäufers G0896034216I4N00

Inhaltsangabe:

David Higgins and James Cregg compile for the first time an all-inclusive collection of key experimental procedures to manipulate Pichia pastoris for gene expression purposes. The methods - developed by world-class researchers in their own laboratories - contain detailed descriptions of available expression strains, as well as vectors and step-by-step instructions for the construction of expression strains and for their use in the production of foreign proteins from shake-flask or fermentor cultures. General procedures for the purification of foreign proteins are also provided, along with protocols for analyzing foreign protein products for glycosylation and subcellular location. Its detailed instructions will permit any researcher in molecular biology to quickly construct an expression strain that will produce a desired foreign protein, and to use this state-of-the-art molecular and cellular biological system successfully in every area of biological research today.

Críticas: In this valuable Methods in Molecular Biology volume, the editors have assembled an international cast of Pichia experts who provide detailed protocols, advice, and theory on recombinant expression in this methylotrophic yeast...a focused writing style that is enjoyable to read. Molecular geneticists are afforded the essential information to achieve success in vector construction, transformation and screening. - The Quarterly Review of Biology

Bibliografische Details

Titel: Pichia Protocols (Methods in Molecular ...
Verlag: Humana Press
Erscheinungsdatum: 1998
Einband: Hardcover
Zustand: Very Good
Zustand des Schutzumschlags: No Jacket