Pichia Protocols

James M Cregg

ISBN 10: 1617375632 / ISBN 13: 9781617375637

Verlag: Humana Press, 2010

Beschreibung

nach der Bestellung gedruckt Neuware - Printed after ordering - Pichia Protocols focuses on recent developments of Pichia pastoris as a recombinant protein production system. Highlighted topics include adiscussion on the use of fermentors to grow Pichia pastoris, information on the O- and N-linked glycosylation, methods for labeling Pichia pastoris expressed proteins for structural studies, and the introduction of mutations in Pichia pastoris genes by the methods of restriction enzyme-mediated integration (REMI).This volume fully updates and expands upon the first edition of Pichia Protocols, and focuses primarily on information that has come to light since its original publication. Each chapter presents cutting-edge and cornerstone protocols for utilizing P. pastoris as a model recomibinant protein production system.

Bestandsnummer des Verkäufers 9781617375637

Bibliografische Details

• Titel: Pichia Protocols
• Verlag: Humana Press
• Erscheinungsjahr: 2010
• Sprache: Englisch
• ISBN-10: 1617375632
• ISBN-13: 9781617375637
• Einband: Taschenbuch
• Auflage: 2. Auflage
• Zustand: Neu

Über diesen Titel

Inhaltsangabe

This book focuses on recent developments of Pichia pastoris as a recombinant protein production system. The volume fully updates and expands upon the first edition, and focuses primarily on information that has come to light since its original publication. Highlighted topics include a discussion on the use of fermentors to grow Pichia pastoris, information on the O- and N-linked glycosylation, methods for labeling Pichia pastoris expressed proteins for structural studies, and the introduction of mutations in Pichia pastoris genes by the methods of restriction enzyme-mediated integration (REMI).

Each chapter presents cutting-edge and cornerstone protocols for utilizing P. pastoris as a model recomibinant protein production system.

Von der hinteren Coverseite

<p>The use of Pichia Pastoris as a recombinant protein system has increased greatly in popularity over the years. In Pichia Protocols 2^nd Edition, the author focuses on new information that has come to light in recent years but repeats key basic methods so that researchers are not absolutely dependent on having both editions in order to utilize the system. The chapters in this indispensable laboratory aid cover a wide range of topics associated with Pichia Pastoris. The use of fermentors to grow Pichia Pastoris and good advice on how to go about the initial processing of material from these cultures is discussed. An extensive discussion on how to formally analyze mutations in essential P. pastoris genes is also presented. The final chapters are for cell biologists who are for cell biologists using the Pichia Pastoris system to investigate protein interactions, or to localize proteins in yeast cells. Pichia Protocols 2^nd Edition, is an essential tool for researchers working with the Pichia Pastoris system</p>