Polydnavirus Histone H4 And Its Role In Insect Immunity: Polydnavirus Histone H4. Dieser Artikel ist nicht verfügbar.Polydnavirus Histone H4 And Its Role In Insect Immunity: Polydnavirus Histone H4

Cotesia plutellae (Braconidae: Hymenoptera) is a solitary endoparasitoid wasp that parasitizes the diamondback moth, Plutella xylostella (L.) (Yponomeutidae: Lepidoptera). For successful parasitization, its symbiotic polydnavirus (CpBV) helps the wasps to attenuate host immune capacity and manipulate host developmental physiology. An analysis of CpBV genome sequence indicated the presence of one putative gene (CpBV-H4) showing a high similarity to histone H4. Histone H4, one of the four histone subunits along with H2A, H2B and H3, forms an eukaryotic nucleosome core. Along with H3, it plays a central role in nucleosome formation. CpBV-H4 showed expression in different tissues in parasitized P. xylostella. Transient expression of CpBV-H4 in nonparasitized P. xylostella resulted in significant impairment of hemocyte-spreading and decreased gene expression of host H4, while knock-down of the transient expression using double-stranded RNA interference recovered the immune capacity and gene expression.

Wael Gad is PhD Researcher, Department of Structure Biology at Vrije University Brussels. Master of Science, Department of Bioresource Sciences, Andong National University, South Korea. B.Sc. of Zoology, Department of Zoology and Marine Biology, Al-Azhar University Assiut, Egypt.