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Inhaltsangabe: Promoter binding analysis has largely been a laborious and inefficient process. With the advent of "ChIP on chip" (Chromatin Immunoprecipitation on Microarrays or Chips) the research community has been given a new, powerful and high throughput option. This book aims to not only introduce the power of this technology but specifically to provide detailed protocols as to how to perform a ChIP on chip experiment. This book will provide the reader a number of benefits: * Detailed explanations of the Chromatin Immunoprecipitation technique * Protocols for performing ChIP on chip experiments * Informatics approaches to identifying promoter regions in genomic DNA * Guidelines for analyzing the data that is obtained from a ChIP on chip experiment * Techniques for methylation analysis of CpG islands using CpG microarrays * Indications of future directions for improvements to the technology including the development of promoter specific arrays, and regulatory region arrays (enhancers and suppressors).
Inhaltsangabe: An overview of the emerging technology of Chromatin Immunoprecipitation on microarrays or chips (ChIP on chip) is provided in this guide to improving the efficiency of the promoter binding analysis of DNA. Detailed explanations of techniques and protocols for performing the ChIP on chip experiments accompany guidelines for analyzing the data that is obtained. Methylation analysis of a CpG island using CpG microarrays allows researchers to develop arrays of thousands of promoter regions without cloning promoters. Discussions of these innovations and future directions for improvement provide researchers essential information for utilizing this new technology.
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