Protein Chromatography: Methods and Protocols

Dermot Walls

ISBN 10: 1493981919 / ISBN 13: 9781493981915

Verlag: Humana Press Inc., 2018

Beschreibung

This item is printed on demand. New copy - Usually dispatched within 5-9 working days 799.

Bestandsnummer des Verkäufers C9781493981915

Bibliografische Details

• Titel: Protein Chromatography: Methods and Protocols
• Verlag: Humana Press Inc.
• Erscheinungsjahr: 2018
• Sprache: Englisch
• ISBN-10: 1493981919
• ISBN-13: 9781493981915
• Einband: Paperback / softback
• Zustand: New

Über diesen Titel

Inhaltsangabe

This second edition expands on the previous edition with new chapters that are suitable for newcomers, as well as more detailed chapters that cover protein stability and storage, avoiding proteolysis during chromatography, protein quantitation methods including immuno-qPCR, and the challenges that scale-up of production poses to the investigator. Many of the chapters also discuss generation and purification of recombinant proteins, recombinant antibody production, and the tagging of proteins as a means to enhance their solubility and simplify their purification on an individual scale or in high-throughput systems. This book also provides readers with chapters that describe not just the more commonly used methods, but also recently developed approaches such as proteomic/mass spectrometric techniques and Lectin-based affinity chromatography. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Protein Chromatography: Methods and Protocols, Second Edition is a valuable resource for anyone who is interested in the field of protein chromatography.