Protein Purification Techniques (Paperback)

Sprache: Englisch

Verlag: Oxford University Press, Oxford, 2001

0199636737 / 9780199636730

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Artikelbeschreibung des Verkäufers

Paperback. Proteins are an integral part of molecular and cellular structure and function and are probably the most purified type of biological molecule. In order to elucidate the structure and function of any protein it is first necessary to purify it. Protein purification techniques have evolved over the past ten years with improvements in equipment control, automation, and separation materials, and the introduction of new techniques such as affinity membranes and expandedbeds. These developments have reduced the workload involved in protein purification, but there is still a need to consider how unit operations linked together to form a purification strategy, which canbe scaled up if necessary. The two Practical Approach books on protein purification have therefore been thoroughly updated and rewritten where necessary. The core of both books is the provision of detailed practical guidelines aimed particularly at laboratory scale purification. Information on scale-up considerations is given where appropriate. The books are not comprehensive but do cover the major laboratory techniques and common sources of protein. Protein Purification Techniquesfocuses on unit operations and analytical techniques. It starts with an overview of purification strategy and then covers initial extraction and clarification techniques. The rest of the book concentrates ondifferent purification methods with the emphasis being on chromatography. The final chapter considers general scale-up considerations. Protein Purification Applications describes purification strategies from common sources: mammalian cell culture, microbial cell culture, milk, animal tissue, and plant tissue. It also includes chapters on purification of inclusion bodies, fusion proteins, and purification for crystallography. A purification strategy that can produce a highly puresingle protein from a crude mixture of proteins, carbohydrates, lipids, and cell debris to is a work of art to be admired. These books (available individually or as a set)are designed to give the laboratory workerthe information needed to undertake the challenge of designing such a strategy. The protein purification Practical Approach books contain detailed practical information on the separation and purification of proteins. Protein Purification Techniques covers unit operations and analytical techniques in detail, while Protein Purification Applications has details of protein purification from a selected number of typical sources. This item is printed on demand. Shipping may be from our Sydney, NSW warehouse or from our UK or US warehouse, depending on stock availability.…

Bestandsnummer des Verkäufers 9780199636730

Titel
Protein Purification Techniques (Paperback)
Autor
Simon Roe
Verlag
Oxford University Press, Oxford
Erscheinungsjahr
2001
Zustand
new
Einband
Paperback
Sprache
Englisch
ISBN-10
0199636737
ISBN-13
9780199636730
Auflage
2. Auflage

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