1588293432 Good+; Hardcover; 2005, Humana Press; Former library copy with standard library markings; Light wear to covers; Library stamps to endpapers; Text pages clean & unmarked; Good binding with straight spine; Orange and yellow covers with title in white lettering; 1016 pages; "The Proteomics Protocols Handbook (Methods in Molecular Biology)," by Jane Jacobs, et al.

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Titel: The Proteomics Protocols Handbook (Methods in Molecular Biology)
Verlag: Humana
Ver�ffentlichungsdatum: 2005
Sprache: Englisch
ISBN-10: 1588293432
ISBN-13: 9781588293435
Bindung: Hardcover
Zustand: Good

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Hands-on researchers describe in step-by-step detail 73 proven laboratory methods and bioinformatics tools essential for analysis of the proteome. These cutting-edge techniques address such important tasks as sample preparation, 2D-PAGE, gel staining, mass spectrometry, and post-translational modification. There are also readily reproducible methods for protein expression profiling, identifying protein-protein interactions, and protein chip technology, as well as a range of newly developed methodologies for determining the structure and function of a protein. The bioinformatics tools include those for analyzing 2D-GEL patterns, protein modeling, and protein identification. All laboratory-based protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

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Proteomics methodology has revolutionized the way that proteins are studied and opened new channels to understanding both cell functions and the cellular changes involved in disease states. In The Proteomics Protocols Handbook, hands-on researchers describe in step-by-step detail a wide range of proven laboratory methods and bioinformatics tools essential for analysis of the proteome. These cutting-edge techniques (73 in all) address such important tasks as sample preparation, 2D-PAGE, gel staining, mass spectrometry, and posttranslational modification. There are also readily reproducible methods for protein expression profiling, identifying protein-protein interactions, and protein chip technology, as well as a range of newly developed methodologies for determining the structure and function of a protein, including novel mass spectrometry and LC-MS techniques, protein array technology, and a variety of structural and functional proteomics techniques needed to determine of the function of newly discovered protein sequences. The use of X-ray crystallography and NMR to determine protein structure is also described. The bioinformatics tools include those for analyzing 2D-GEL patterns, protein modeling, and protein identification. All laboratory-based protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.<br> Timely and highly practical, The Proteomics Protocols Handbook offers molecular biologists, protein chemists, clinical and medicinal chemists, biochemists, and microbiologists a comprehensive collection of today's best tools for understanding not only the function of individual proteins in the cell, but also for identifying new therapeutic targets.

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Thank you for taking an interest in one of our books. We are a mail-order only bookstore located in Bellingham, WA specializing in modern out-of-print books of all genres.

If you are an international customer, we ship internationally by UPS Worldwide Expedited. The average delivery time is 1 to 2 weeks, and each shipment can be fully tracked at www.ups.com. For international shipments, it is important to provide a valid phone number with your order. Most international shipments are delivered wit...

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