Part I: Stem Cells and Migration: An Overview
1. Trafficking of Stem Cells
Claire Magnon, Daniel Lucas, and Paul S. Frenette
2. Migratory Strategies of Normal and Malignant Stem Cells
David A. Hess and Alison L. Allan
Part II: Stem Cells Identification and Microscopic Technologies to Track Transplanted Stem Cells In Vivo
3. Hematopoietic Stem Cell Characterization and Isolation
Lara Rossi, Grant A. Challen, Olga Sirin, Karen Kuan-Yin Lin, and Margaret A. Goodell
4. Isolation and Characterization of Adult Neural Stem Cells
Florian A. Siebzehnrubl, Vinata Vedam-Mai, Hassan Azari, Brent A. Reynolds, and Loic P. Deleyrolle
5. Magnetic Resonance Imaging of Stem Cell Migration
Eva Syková Pavla Jendelová, and Vít Herynek
6. Imaging of Schwann Cells In Vivo
Rahul Kasukurthi and Terrence M. Myckatyn
7. Imaging of Embryonic Stem Cell Migration In Vivo
Andrew S. Lee and Joseph C. Wu
Part III: Stem Cell Migration During Development
8. In Vivo Germ Line Stem Cell Migration: A Mouse Model
Brian Dudley and Kathleen Molyneaux
9. Live Microscopy of Neural Stem Cell Migration in Brain Slices
Jin-Wu Tsai and Richard B. Vallee
10. Whole Embryo Imaging of Hematopoietic Cell Emergence and Migration
Michael J. Ferkowicz and Mervin C. Yoder
11. Stem Cell Migration: A Zebrafish Model
Pulin Li and Leonard I. Zon
12. Imaging Pluripotent Cell Migration in Drosophila
Michael J. Murray and Robert Saint
Part IV: Adult Hematopoietic Stem Cell Migration
13. Adhesion, Migration, and Homing of Murine Hematopoietic Stem Cells and Progenitors
Jose A. Cancelas
14. Methods to Analyze the Homing Efficiency and Spatial Distribution of Hematopoietic Stem and Progenitor Cells and Their Relationship to the Bone Marrow Endosteum and Vascular Endothelium
Jochen Grassinger and Susan K. Nilsson
15. Imaging Hematopoietic Stem Cells in the Marrow of Long Bones In Vivo
Anja Köhler, Hartmut Geiger, and Matthias Gunzer
Part V: Non Hematopoietic Stem Cell Migration
16. Studies of Adult Neural Stem Cell Migration
Adam C. Puche and Serena Bovetti
17. Dissecting Mesenchymal Stem Cell Movement: Migration Assays for Tracing and Deducing Cell Migration
Erika L. Spaeth and Frank Marini
18. Epithelial Stem Cells
Kyle M. Draheim and Stephen Lyle
Part VI: Pathways Implicated in Stem Cell Migration
19. Pathways Implicated in Stem Cell Migration: The SDF-1/CXCR4 Axis
Yaron Vagima, Kfir Lapid, Orit Kollet, Polina Goichberg, Ronen Alon, and Tsvee Lapidot
20. The Role of Receptor Tyrosine Kinases in Primordial Germ Cell Migration
Louise Silver-Morse and Willis X. Li
21. Rho GTPases in Hematopoietic Stem/Progenitor Cell Migration
Wei Liu, Yuxin Feng, Xun Shang, and Yi Zheng
The migration of stem cells has been found to be critical during early development for the organization of the embryonic body as well as during adult life with tissue homeostasis and regeneration of organ function. Due to the low frequency of these cells in vivo, problems in identifying and prospectively purifying tissue specific stem cells near homogeneity, and, most importantly, a lack of adequate technologies and protocols to study stem cell migration in vivo, this vital research has been quite difficult until recently. In Stem Cell Migration: Methods and Protocols, experts in the field compile and highlight the standard and novel techniques that allow the studying of the migration of stem cells in one succinct manual. Including protocols on germ, neuronal, and hematopoietic stem cells, during development and adulthood with a clear emphasis on in vivo technologies, the volume also extends its coverage to in vitro approaches toward several developmentally-conserved signaling pathways. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and convenient,
Stem Cell Migration: Methods and Protocols provides key, state-of the art information on experimental techniques for studying stem cell migration both at a cellular and molecular level in development, regeneration, and disease.
