1. Expression and Purification of Human Mitochondrial Intramembrane Protease PARL
Elena Arutyunova, Laine Lysyk, Melissa Morrison, Cory Brooks, and M. Joanne Lemieux
2. Reconstitution of Detergent-Solubilized Membrane Proteins into Proteoliposomes and Nanodiscs for Functional and Structural Studies
Kerry M. Strickland, Kasahun Neselu, Arshay J. Grant, Carolann L. Espy, Nael A. McCarty, and Ingeborg Schmidt-Krey
3. Biochemical Characterization of GPCR-G Protein Complex Formation
Filip Pamula and Ching-Ju Tsai
4. Electrophysiological Approaches for the Study of Ion Channel Function
Guiying Cui, Kirsten A. Cottrill, and Nael A. McCarty
5. Isothermal Titration Calorimetry of Membrane Proteins
Han N. Vu, Alan J. Situ, and Tobias S. Ulmer
6. Atomic Force Microscopy Reveals Membrane Protein Activity at the Single Molecule Level
Kanokporn Chattrakun, Katherine G. Schaefer, Lucas S. Chandler, Brendan P. Marsh, and Gavin M. King
7. Structure Determination of Membrane Proteins Using X-Ray Crystallography
Evan Billings, Karl Lundquist, Claire Overly, Karthik Srinivasan, and Nicholas Noinaj
8. Studying Membrane Protein Structures by MicroED
Michael W. Martynowycz and Tamir Gonen
9. Single-Particle Cryo-EM of Membrane Proteins
Dovile Januliene and Arne Moeller
10. Helical Membrane Protein Crystallization in the New Era of Electron Cryo-Microscopy
Mary D. Hernando, Joseph O. Primeau, and Howard S. Young
11. NMR Spectroscopic Studies of Ion Channels in Lipid Bilayers: Sample Preparation Strategies Exemplified by the Voltage Dependent Anion Channel
Robert Silvers and Matthew Eddy
12. Preparation of a Deuterated Membrane Protein for Small-Angle Neutron Scattering
Yuqi Wu, Kevin L. Weiss, and Raquel L. Lieberman
13. Preparing Membrane Proteins for Simulation Using CHARMM-GUI
Yupeng Li, Jinchan Liu, and James C. Gumbart
14. Coarse-Grained Molecular Dynamics Simulations of Membrane Proteins: A Practical Guide
William Glass, Jonathan W. Essex, Franca Fraternali, James Gebbie-Rayet, Irene Marzuoli, Marley L. Samways, Philip C. Biggin, and Syma Khalid
15. Continuous Constant pH Molecular Dynamics Simulations of Transmembrane Proteins
Yandong Huang, Jack A. Henderson, and Jana Shen
16. Molecular Dynamics-Based Thermodynamic and Kinetic Characterization of Membrane Protein Conformational Transitions
Dylan Ogden and Mahmoud Mora
This book examines detailed experimental and computational approaches for the analysis of many aspects vital to the understanding of membrane protein structure and function. Readers will receive guidance on the selection and use of methods for over-expression and purification, tools to characterize membrane proteins within different phospholipid bilayers, direction on functional studies, and approaches to determine the structures of membrane proteins. Detailed experimental steps for specific membrane proteins with critical notes allow the protocols to be modified to different systems. Written for the highly successful <i>Methods in Molecular Biology</i> series, chapters include the kind of practical information and implementation advice that leads to excellent, reproducible results. <div><br></div><div>Authoritative and up-to-date, <i>Structure and Function Studies of Membrane Proteins</i> serves as an ideal guide for biologists, biochemists, and biophysicists striving to further understand these essential proteins and their many biological roles.</div>
