TALENs : Methods and Protocols

Ralf Kühn

ISBN 10: 1493929313 / ISBN 13: 9781493929313

Verlag: Springer New York, Springer US, 2015

Beschreibung

Druck auf Anfrage Neuware - Printed after ordering - This volume provides a comprehensive collection of protocols on new technology across various model organisms. Chapters describe species-specific methods to generate new mutants the content is completed by chapters on natural TAL effectors, TAL element DNA binding principles, TALEN target site prediction, and methods for the efficient construction of TALEN coding regions. While addition chapter focus on the application of TALEN as sequence-specific nucleases and TAL based gene activators or inhibitors and the visualization of chromatin dynamics in live cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, TALENs: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Bestandsnummer des Verkäufers 9781493929313

Bibliografische Details

• Titel: TALENs : Methods and Protocols
• Verlag: Springer New York, Springer US
• Erscheinungsjahr: 2015
• Sprache: Englisch
• ISBN-10: 1493929313
• ISBN-13: 9781493929313
• Einband: Buch
• Zustand: Neu
• Abmessungen: 260B x 183 H x 21 T Millimeter Breite: 260 mm, Höhe: 183 mm, Tiefe: 21 mm
• Gewicht: 808 Gramm

Über diesen Titel

Inhaltsangabe

​This volume provides a comprehensive collection of protocols on new technology across various model organisms.  Chapters describe species-specific methods to generate new mutants the content is completed by chapters on natural TAL effectors, TAL element DNA binding principles, TALEN target site prediction, and methods for the efficient construction of TALEN coding regions. While addition chapter focus on the application of TALEN as sequence-specific nucleases and TAL based gene activators or inhibitors and the visualization of chromatin dynamics in live cells.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, TALENs: Methods and Protocols aims to ensure successful results in the further study of this vital field.